Briefly describe an in vitro experiment that differentiate between the Arp2/3 complex’ ability to associate with both the side of the ‘mother’ actin filament and with the barbed end of the ‘mother’ actin filament. Your experiment should take advantage of capping protein (binds and blocks barbed ends), and direct visualization of actin filaments.
Briefly describe an in vitro experiment that differentiate between the Arp2/3 complex’ ability to associate with both the side of the ‘mother’ actin filament and with the barbed end of the ‘mother’ actin filament. Your experiment should take advantage of capping protein (binds and blocks barbed ends), and direct visualization of actin filaments.
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- Briefly describe an in vitro experiment that differentiate between the Arp2/3 complex’ ability to associate with both the side of the ‘mother’ actin filament and with the barbed end of the ‘mother’ actin filament. Your experiment should take advantage of capping protein (binds and blocks barbed ends), and direct visualization of actin filaments.
- Draw diagrams of actin filaments (simple lines are okay) that indicate the possible outcomes of your experiment.
- describe your interpretation of the possible outcomes for the mechanism of Arp2/3 complex-mediated branch formation
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