Briefly describe an in vitro experiment that differentiate between the Arp2/3 complex’ ability to associate with both the side of the ‘mother’ actin filament and with the barbed end of the ‘mother’ actin filament. Your experiment should take advantage of capping protein (binds and blocks barbed ends), and direct visualization of actin filaments.Draw diagrams of actin filaments (simple lines are okay) that indicate the possible outcomes of your experiment.describe your interpretation of the possible outcomes for the mechanism of Arp2/3 complex-mediated branch formation

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Answered: Briefly describe an in vitro experiment…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Briefly describe an in vitro experiment that differentiate between the Arp2/3 complex’ ability to associate with both the side of the ‘mother’ actin filament and with the barbed end of the ‘mother’ actin filament. Your experiment should take advantage of capping protein (binds and blocks barbed ends), and direct visualization of actin filaments.
Draw diagrams of actin filaments (simple lines are okay) that indicate the possible outcomes of your experiment.
describe your interpretation of the possible outcomes for the mechanism of Arp2/3 complex-mediated branch formation

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