Introduction to Experiment 2: Protein Digestion by PepsinIn this experiment, we will look at for evidence of protein đigestion using albumin, a protein, andBiuret reagent Biuret reagent is used to test for the presence of proteins, or more specifically,peptide bonds. When long polypeptide chains are present the solution will turn violet, and whenshorter polypeptide chains are present the solution will turn a lighter pink color.1. What is/are the end producus) of protein digestion?2. What is the purpose of the enzyme pepsin?3. If protein digestion bas occurred, will the solution be violet or pink (choose one)? Explain.Hypotheses/Predictions (See Methods section for details):1.What color do you expect the solution to turn when the P tubes are mixed with Biuretsolution and why?PIP2P3P42. The least protein digestion (or none at allD will take place in tubes (P1, P2, P3, and/or P4)?Why?What color will these tubes be after the Biuret test?2.Lab 8will have more protein digestion than tube3. Of the remaining two tubes, tubeWhy? Introduction to Experiment 1: Starch Digestion by AmylaseIn this experiment, we will look for evidence of starch digestion by testing solutions for the presence of starchand sugars. When starch s present the lodine in Lugols solution turns the solution black, blue, or darkbeown If starch is not present, lodine will dye the solution a yellow color. Benedict's reagent reacts withfreducing sugars, Includinggucose, resulting in a green, orange, or red color. If sugars are not present,Benedict s reagent remains a light blue color.1. What is/are the end product(s) of starch digestion?2. What is the name of the enzyme that digests starch (amylose)?Hypotheses Predictions (See Methods section for details)1. When mixed with Lugols solution, test tube Al (amylase only) will have what color andwhy?2. When mixed with Benedict's solution, test tube Al (amylase only) will have what color andwhy?3. When mixed with Lugols solution, test tube A2 (starch only) will have what color and why?4. When mixed with Benedict's solution, test tube A2 (starch only) will have what color andwhy5. When mixed with Lugol's solution, test tube A3 (glucose only) will have what color andwhy?6. When mixed with Benedict's solution, test tube A3 (glucose only) will have what color andwhy?7. When mixed with Lugol s solution, test tube A4 (amylase and starch) will have what colorand why?Anatomy and Physiology II, BIOL 2200Lab 88. When mixed with Benedict's solution, test tube A4 (amylase and starch) will have what colorand why?

Proteins are made up of amino acids linked together by peptide bonds. When proteins are digested…

Introduction to Experiment 2: Protein Digestion by Pepsin In this experiment, we will look at for evidence of protein digestion using albumin, a protein, and Biuret reagent. Biuret reagent is used to test for the presence of proteins, or more specifically, peptide bonds. When long polypeptide chains are present the solution will turn violet, and when shorter polypeptide chains are present the solution will turn a lighter pink color. 1. What is/are the end product(s) of protein digestion? 2. What is the purpose of the enzyme pepsin? 3. If protein digestion has occurred, will the solution be violet or pink (choose one)? Explain. Hypotheses/Predictions (See Methods section for details): 1. What color do you expect the solution to turn when the P tubes are mixed with Biuret

Introduction to Experiment 2: Protein Digestion by Pepsin In this experiment, we will look at for evidence of protein digestion using albumin, a protein, and Biuret reagent. Biuret reagent is used to test for the presence of proteins, or more specifically, peptide bonds. When long polypeptide chains are present the solution will turn violet, and when shorter polypeptide chains are present the solution will turn a lighter pink color. 1. What is/are the end product(s) of protein digestion? 2. What is the purpose of the enzyme pepsin? 3. If protein digestion has occurred, will the solution be violet or pink (choose one)? Explain. Hypotheses/Predictions (See Methods section for details): 1. What color do you expect the solution to turn when the P tubes are mixed with Biuret

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Drugs are small molecules that often occur in either a neutral or a charged form, depending on pH. For example, aspirin can be either neutral or lose a proton to be negatively charged. When aspirin is charged, it does not diffuse well into the body. pH varies in the gastrointestinal track. Since the stomach has a low pH (pH=3), it has a larger concentration of neutral aspirin (10-3 M), while the small intestine (pH=6) has a smaller neutral aspirin concentration (10-6 M). For the following, assume that the neutrally charged aspirin molecule has a diffusion coefficient through the cell of 1 x 10-6 cm2/s. C) We’d like to figure out where in the body aspirin is absorbed as determined by its diffusion rate. If the small intestine has a surface area 50 times that of the stomach, what is the ratio of the diffusion rates in the stomach compared to the small intestine? D) In which part of the GI tract is most of the aspirin absorbed?
Uhe organism at a rate adequate to 45. Which of the following.BEST describes the biological value of a protein? A. The percentage of ingested protein utilized for protein synthesis in the body 8. The percentagé of ingested protein/nitrogen absorbed into the circulation C. The percentsge of ingested protein/nitrogen in the body D. The gain ir body weight per gram of protein ingested Use the structure of the peptide below to answer the next five items. 46. Howmany amino acids are there in this oligopeptide? A Seven A. Light Nine D. Ten A7. How many peptide bons are present? A Seven B. Eight C Nine D. Ten 48. What is the N-terminus amino acid? A Glutamic acid B. Arginine C. Serine D Phenylalanine E None of the above 49. What is Uhe Č terminus amino acid? A. Phenylalanine B. Threonine C. Serine D. Arginine E None of the above 50. How many achiral ami0o acids are present in this amino acid chain? A One B. Two C. Three D. Four Previous Question Page 4 of 4 Next
Which of the following is true regarding proteins A. Absorbance of proteins is directly proportional to the molecular weights B.Proteins react with biuret reagent because of their side chains C. two out of the twenty amino acids can absorb at 280nm D. In proteins the average weight per amino acid residue always varies
Test your understanding: If you would like to make a 100 ml, 0.8% agarose geland you are given TBE buffer with a 50X stock concentration, how much agarosepowder, 50X TBE buffer, and diluent will you need?
9) The diagram below represents events involved as energy is ultimately released from food. Food molecule within digestive system xxx X-XX cool wat Food molecule outside of body x-xx XX-X A) 1 B) 2 The enzyme amylase will affect the breakdown of carbohydrates, but it will not affect the break- down of proteins. The ability of an enzyme molecule to interact with specific molecules is most directly determined by the (1) shapes of the molecules involved (2) number of molecules involved (3) sequence of bases present in ATP (4) amount of glucose present in the cell 6 A^XXXX X Which row in the table below best represents the chain of Xs and letters A and Bin the diagram? X-X-X-X-X-X-X A and B (1) nutrient antibodies (2) nutrient enzymes (3) hemoglobin wastes (4) hemoglobin hormones C) 3 In the human body, oxygen is absorbed by the lungs and nutrients are absorbed by the small intestine. In a single-celled organism, this absorption directly involves the (1) nucleus (2) chloroplasts Portion of…
(a) What is the charge of bile acids in the small intestine, where the pH is 7–8? (b) According to one hypothesis, bile acids are toxic to bacteria and so could help limit their growth in the intestine. Propose a mechanism for this bactericidal activity.
You obtain two segments of dialysis tubing with large pores. You fill two large beakers with distilled water. In beaker A, you place a dialysis bag (bag A) containing a 1% solution of a protein called Tiny. In beaker B, you place a dialysis bag (bag B) containing a 1% solution of a protein called Dinky. After two hours, you recover the bag contents and the beaker contents and perform a biuret assay. The results of the assay are shown below. What can you conclude about the relative sizes of Tiny and Dinky?
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1. Discuss various methods of extraction of lipids from food/feed material. 2. What are various factors which affect lipid extraction? 3. Will the lipid extraction in this method be considered representative of all available lipids in your sample? If not, name those lipids which are not present in the extract obtained by this method. Discuss the basis of your conclusion.
Chapter 6: PROTEINS What are the roles of proteins in the body. a) What is the basic unit of proteins? b.)What are Essential Amino Acids? c.)How many essential and how many non-essential amino acids do we have? Explain why protein formation is an all or nothing event? How are the amino acids recycled? What is protein denaturation? Is denaturation harmful or beneficial? Explain your answer a.) How proteins digested and absorbed in the body? b.) What happens to an amino acid if it consumed in large amounts? Is it all absorbed? c.) What happens to proteins once absorbed into the body’s cells? What are they initially used for? 7.) What is protein quality? Give examples of high and low quality proteins. 8.) What happens to excess amino acids? 9.) What is a limiting amino acid? Explain what happens if an amino acid is limiting. 10.) a.)Does everyone need to eat animal protein? b.) what are some health benefits of a vegetarian diet? c.) What are the nutrients that a commonly…
multiple choice question: what R groups of the amino acids would most likely be found in the core of globular protein in an aqueous solution? (a) Ser & Thr (b) Ile & Phe (c) Glu & Gln (d) Asp & Lys (e) Trp & His
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