intracellular concentration of P. The increase in P, concentration would drive the unfavorable phosphorylation of glucose b Is increasing the P concentration a reasonable way to couple ATP hydrolysis and glucose phosphorylation? O No. The phosphate salts of divalent cations would be present in excess and precipitate out. Yes. The extra ATP hydrolysis would provide enough free energy to drive the phosphorylation reaction. Yes. Increasing the concentration of P, would decrease K'e and shift equilibrium to the right. No. The extra P would give a negative AG, but would give a positive AG. In hepatocytes, the enzyme glucokinase catalyzes the ATP-coupled phosphorylation of glucose. Glucokinase binds both A and glucose, forming a glucose-ATP-enzyme complex. The enzyme then transfers the phosphoryl group directly from AT

intracellular concentration of P. The increase in P, concentration would drive the unfavorable phosphorylation of glucose b Is increasing the P concentration a reasonable way to couple ATP hydrolysis and glucose phosphorylation? O No. The phosphate salts of divalent cations would be present in excess and precipitate out. Yes. The extra ATP hydrolysis would provide enough free energy to drive the phosphorylation reaction. Yes. Increasing the concentration of P, would decrease K'e and shift equilibrium to the right. No. The extra P would give a negative AG, but would give a positive AG. In hepatocytes, the enzyme glucokinase catalyzes the ATP-coupled phosphorylation of glucose. Glucokinase binds both A and glucose, forming a glucose-ATP-enzyme complex. The enzyme then transfers the phosphoryl group directly from AT

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

Coupling ATP hydrolysis to glucose phosphorylation makes thermodynamic sense, but consider how the coupling might
take place.
Given that coupling requires a common intermediate, one conceivable mechanism is to use ATP hydrolysis to raise the
intracellular concentration of P,. The increase in P, concentration would drive the unfavorable phosphorylation of glucose by P.
Is increasing the P concentration a reasonable way to couple ATP hydrolysis and glucose phosphorylation?
No. The phosphate salts of divalent cations would be present in excess and precipitate out.
Yes. The extra ATP hydrolysis would provide enough free energy to drive the phosphorylation reaction.
Yes. Increasing the concentration of P, would decrease K'e and shift equilibrium to the right.
No. The extra P, would give a negative AG, but would give a positive AG.
In hepatocytes, the enzyme glucokinase catalyzes the ATP-coupled phosphorylation of glucose. Glucokinase binds both ATP
and glucose, forming a glucose-ATP-enzyme complex. The enzyme then transfers the phosphoryl group directly from ATP
to glucose.
In hepatocytes, the enzyme glucokinase catalyzes the ATP-coupled phosphorylation of glucose. Glucokinase binds both ATP
and glucose, forming a glucose-ATP-enzyme complex. The enzyme then transfers the phosphoryl group directly from ATP
to glucose.
Select the advantages of phosphoryl group transfer compared to hydrolysis and subsequent phosphorylation?
O Reaction intermediates do not need to be present in excess.
Glucokinase increases the transition state energy, favoring glucose phosphorylation.
O ATP hydrolysis is thermodynamically unfavorable compared to group transfer.
The process takes advantage of the high phosphoryl group transfer potential of ATP.

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Coupled reactions occur where a nonspontaneous reaction is enabled by coupling it to a spontaneous reaction. This approach is common in biological settings. Determine if ATP could be generated by this biochemical reaction. You have calculated that cell potential is +0.637V. An example of a coupled reaction is the first step of glycolysis, the phosphorylation of glucose to form glucose-6-phosphate shown below. The net ∆Gº for this reaction is
Coupled reactions occur where a nonspontaneous reaction is enabled by coupling it to a spontaneous reaction. This approach is common in biological settings. Determine if ATP could be generated by this biochemical reaction. You have calculated that cell potential is +0.637V. An example of a coupled reaction is the first step of glycolysis, the phosphorylation of glucose to form glucose-6-phosphate shown below. kJ/mol The net AG° for this reaction is 1 2 3 н он H. H- H- H H H H 4 6. H. glucose phosphate anion glucose-6-phosphate AG = +14.0 kJ/mol 7 8 9. АТР ADP phosphate anion AG = -30.5 kJ/mol +/- LO
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Is increasing the P; concentration a reasonable way to couple ATP hydrolysis and glucose phosphorylation? Yes. Increasing the concentration of P; would decrease K'eq and shift equilibrium to the right. No. The phosphate salts of divalent cations would be present in excess and precipitate out. No. The extra P; would give a negative AG", but would give a positive AG. Yes. The extra ATP hydrolysis would provide enough free energy to drive the phosphorylation reaction.
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