INSTRUCTION: EXPLAIN THOROUGHLY THE TWO THEORIES INVOLVED IN GRAM STAINING (CELL WALL AND LIPIDS THEORIES) IN RELATION TO THE TWO STATEMENTS BELOW. In THEORY OF CELL WALL, Gram-positive bacteria have heavy peptidoglycan, which helps decolorizer (alcohol) to dehydrate the gram-positive cell wall and traps the crystal violet complex inside the cell wall and maintains the purple color of crystal violet.  In THEORY OF LIPIDS, Gram-negative bacteria have high lipid amount (10-15%) in the cell wall, which makes decolorizer (alcohol) easily to remove the crystal violet complex, and then colorless cell wall is stained with safranin and appears pink/red color.

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Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.