INSTRUCTION: EXPLAIN THOROUGHLY THE TWO THEORIES INVOLVED IN GRAM STAINING (CELL WALL AND LIPIDS THEORIES) IN RELATION TO THE TWO STATEMENTS BELOW. In THEORY OF CELL WALL, Gram-positive bacteria have heavy peptidoglycan, which helps decolorizer (alcohol) to dehydrate the gram-positive cell wall and traps the crystal violet complex inside the cell wall and maintains the purple color of crystal violet. In THEORY OF LIPIDS, Gram-negative bacteria have high lipid amount (10-15%) in the cell wall, which makes decolorizer (alcohol) easily to remove the crystal violet complex, and then colorless cell wall is stained with safranin and appears pink/red color.
INSTRUCTION: EXPLAIN THOROUGHLY THE TWO THEORIES INVOLVED IN GRAM STAINING (CELL WALL AND LIPIDS THEORIES) IN RELATION TO THE TWO STATEMENTS BELOW. In THEORY OF CELL WALL, Gram-positive bacteria have heavy peptidoglycan, which helps decolorizer (alcohol) to dehydrate the gram-positive cell wall and traps the crystal violet complex inside the cell wall and maintains the purple color of crystal violet. In THEORY OF LIPIDS, Gram-negative bacteria have high lipid amount (10-15%) in the cell wall, which makes decolorizer (alcohol) easily to remove the crystal violet complex, and then colorless cell wall is stained with safranin and appears pink/red color.
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter2: Basic Hematology
Section2.7: Preparing And Staining A Blood Smear
Problem 16RQ
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INSTRUCTION: EXPLAIN THOROUGHLY THE TWO THEORIES INVOLVED IN
In THEORY OF CELL WALL, Gram-positive bacteria have heavy peptidoglycan, which helps decolorizer (alcohol) to dehydrate the gram-positive cell wall and traps the crystal violet complex inside the cell wall and maintains the purple color of crystal violet.
In THEORY OF LIPIDS, Gram-negative bacteria have high lipid amount (10-15%) in the cell wall, which makes decolorizer (alcohol) easily to remove the crystal violet complex, and then colorless cell wall is stained with safranin and appears pink/red color.
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