In this qPCR plot, which sample had theleast abundant starting amount of DNA? The image depicts a series of amplification plots from a quantitative PCR (qPCR) experiment. The graph shows several sigmoidal curves representing the amplification of different DNA samples or targets. The x-axis is labeled "cycle number," ranging from 4 to 40, while the y-axis is labeled "relative fluorescence," ranging from 0 to 1.4. This fluorescence indicates the amount of DNA product present, as measured by a fluorescent reporter.Three key points, labeled A, B, and C, are marked with arrows along the cycle number axis at approximately 16, 28, and 32, respectively. These points likely represent the cycle thresholds (Ct values) where the fluorescence reaches a certain threshold, indicating detectable levels of DNA.The curves demonstrate a characteristic exponential phase, where DNA is being amplified, followed by a plateau phase. The spacing of these curves suggests differences in initial DNA concentration among the samples, with the curve reaching point A likely having the highest initial concentration, and the curve reaching point C the lowest.This plot is typically used to analyze and compare the expression levels of genes or quantify DNA samples in various conditions.

Answered: In this qPCR plot, which sample had the…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

In this qPCR plot, which sample had the
least abundant starting amount of DNA?

The image depicts a series of amplification plots from a quantitative PCR (qPCR) experiment. The graph shows several sigmoidal curves representing the amplification of different DNA samples or targets. The x-axis is labeled "cycle number," ranging from 4 to 40, while the y-axis is labeled "relative fluorescence," ranging from 0 to 1.4. This fluorescence indicates the amount of DNA product present, as measured by a fluorescent reporter.

Three key points, labeled A, B, and C, are marked with arrows along the cycle number axis at approximately 16, 28, and 32, respectively. These points likely represent the cycle thresholds (Ct values) where the fluorescence reaches a certain threshold, indicating detectable levels of DNA.

The curves demonstrate a characteristic exponential phase, where DNA is being amplified, followed by a plateau phase. The spacing of these curves suggests differences in initial DNA concentration among the samples, with the curve reaching point A likely having the highest initial concentration, and the curve reaching point C the lowest.

This plot is typically used to analyze and compare the expression levels of genes or quantify DNA samples in various conditions.

Expert Solution

Step 1: Introduction

qPCR(Quantitative-Polymerase Chain Reaction): It is a technique applied to measure the amount of PCR product. This technique is also referred to as reverse-transcriptase-PCR.

The detection of PCR product in qPCR is possible by using a fluorescent reporter molecule in each reaction which yields increased fluorescence with an increasing amount of PCR product. For this purpose, DNA-binding dyes and fluorescent sequenced labelled primers/probes are employed. The thermal cyclers are designed with fluorescence detection modules in order to track the fluorescence signal when amplification process starts. The fluorescence which is measured is directly proportional to the amount of DNA present. With the change in fluorescence over the time, the amount of DNA produced in each cycle ca be determined.