In this experiment, we will investigate lipid digestion. Remember that lipids include fats, so the two terms are often used interchangeably. To do this, we will use litmus cream, which is a mixture of cream and litmus. Cream contains triglycerides (neutral fats). Litmus is a pH indicator. Purple indicates a neutral pH. Pink indicates an acidic pH. Blue indicates a basic pH. How do bile salts act on a mixture of fat and water? ________________________________________________ __________________________________________________________________________________________ What is/are the end product(s) of fat digestion? _____________________________________________________________________ Is either of these end products acidic? If so, which one? ____________________________________________________________
Introduction to Experiment 3: Lipid Digestion by Lipase
In this experiment, we will investigate lipid digestion. Remember that lipids include fats, so the two terms are often used interchangeably. To do this, we will use litmus cream, which is a mixture of cream and litmus. Cream contains triglycerides (neutral fats). Litmus is a pH indicator. Purple indicates a neutral pH. Pink indicates an acidic pH. Blue indicates a basic pH.
- How do bile salts act on a mixture of fat and water? ________________________________________________
__________________________________________________________________________________________
- What is/are the end product(s) of fat digestion? _____________________________________________________________________
- Is either of these end products acidic? If so, which one? ____________________________________________________________
Hypotheses/Predictions (See Methods section for details): :
- Which of the tubes do you expect will have the most digestion of fats? The next most digestion of fats? No digestion of fats? Explain. ____________________________________________________________________________________________
- Rank the tubes in order of predicted acidity at the end of the experiment.
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Most Acidic |
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Least Acidic |
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Tube number |
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- What color do you expect each tube to be after the hour long incubation period?
L3 _____________________________________________________________________________________________________________________
L4 _____________________________________________________________________________________________________________________
L5 _____________________________________________________________________________________________________________________
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