Digestive Enzymes Digestive enzymes are protein-based biological catalysts that have important roles in our lives. They help remove stains from our clothing, turn milk into cheese, and are responsible for turning our dinner into usable fuel for our bodies. Enzymes, however, do not work well universally. Some are meant to work at high temperatures, others at low temperatures. They may work best in acidic conditions or neutral conditions. In this activity, the optimal conditions for two different digestive enzymes will be considered. Lipase is made in the pancreas and breaks down lipids in the small intestine. Pepsin breaks down proteins in the stomach. triglycerides polypeptides Enzymes and Cellular Regulation Rate of reaction a. pepsin b. lipase lipase pepsin 6 glycerol + fatty acids Effect of pH on Enzyme Activity PH small peptides + amino acids 8 10 12 14 Pepsin (stomach) -Lipase 1. In which body organ is pepsin active? 2. In which body organ is pancreatic lipase active? 3. For each enzyme, determine the pH that best represents the environment in which the enzyme is most active.
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
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