In the experiment, the TLC plate was submerged in toluene ethylacetate and had spots of cinnamon leaf oil, cinnamon aldehyde, and cinnamon bark oil. 9 spots total were seen under the UV light, but different spots appeared following a vanillin stain. Why is this so and what does this mean?

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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So I completing a lab report and one of the sections is on TLC analysis. In the experiment, the TLC plate was submerged in toluene ethylacetate and had spots of cinnamon leaf oil, cinnamon aldehyde, and cinnamon bark oil. 9 spots total were seen under the UV light, but different spots appeared following a vanillin stain. Why is this so and what does this mean?

Table 1. UV rf values
lane 1
lane 2
lane 3
0.63
0.37
0.41
0.31
0.23
0.14
0.10
0.08
0.06
Table 2. Vanillin stained rf values
lane 1
color
lane 2
color
lane 3
color
0.84
blue
0.63
pink
0.37
blue
0.41
brown
0.31
white
0.23
green
0.18
green
0.14
pink
0.10
yellow
0.08
yellow
0.06
pink
Transcribed Image Text:Table 1. UV rf values lane 1 lane 2 lane 3 0.63 0.37 0.41 0.31 0.23 0.14 0.10 0.08 0.06 Table 2. Vanillin stained rf values lane 1 color lane 2 color lane 3 color 0.84 blue 0.63 pink 0.37 blue 0.41 brown 0.31 white 0.23 green 0.18 green 0.14 pink 0.10 yellow 0.08 yellow 0.06 pink
type used was not mentioned. The mobile phase was m
also visualized using UV light. (REFRENCE)
a.
b. CE
Figure 2. (a) TLC plate under UV light, (b)TEC plate
leaf, CA is cinnamaldehyde, CB is cinnamon bark
Transcribed Image Text:type used was not mentioned. The mobile phase was m also visualized using UV light. (REFRENCE) a. b. CE Figure 2. (a) TLC plate under UV light, (b)TEC plate leaf, CA is cinnamaldehyde, CB is cinnamon bark
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