Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
In the enolase reaction, the Lys 345 and Glu 211 residues participate in the reaction to convert 2-phosphoglycerate (2-PGA) to phosphoenolpyruvate (PEP). Based on the reaction scheme shown in the figure below, which statement below best describes the roles of Lys 345 and Glu 211 in the enolase reaction? Lys345 Mg2 -H Glu211 Enolate intermediate NH3 Mg Mg OH 0=C H₂O NH₂ 01P10 Mg CH₂

In the enolase reaction, the Lys 345 and Glu 211 residues participate in the reaction to convert 2-phosphoglycerate (2-PGA) to phosphoenolpyruvate (PEP). Based on the reaction scheme shown in the figure below, which statement below best describes the roles of Lys 345 and Glu 211 in the enolase reaction? Lys345 Mg2 -H Glu211 Enolate intermediate NH3 Mg Mg OH 0=C H₂O NH₂ 01P10 Mg CH₂

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
t In the enolase reaction, the Lys 345 and Glu 211 residues participate in the reaction to convert 2-phosphoglycerate (2-PGA) to phosphoenolpyruvate (PEP). Based on the reaction scheme shown in the figure below, which statement below best describes the roles of Lys 345 and Glu 211 in the enolase reaction? Lys345 Mg² Mg O=P-OH -H O-H Glu211 Enolate intermediate NH3 Mg Mg 01P-O -H H₂O NH3 Mg Mg FCH₂ 0=
Transcribed Image Text:t In the enolase reaction, the Lys 345 and Glu 211 residues participate in the reaction to convert 2-phosphoglycerate (2-PGA) to phosphoenolpyruvate (PEP). Based on the reaction scheme shown in the figure below, which statement below best describes the roles of Lys 345 and Glu 211 in the enolase reaction? Lys345 Mg² Mg O=P-OH -H O-H Glu211 Enolate intermediate NH3 Mg Mg 01P-O -H H₂O NH3 Mg Mg FCH₂ 0=
Lys 345 functions as general base and Glu 211 functions as a general acid. Lys 345 functions as a electrophile and Glu 211 functions as a nucleophile. Glu 211 functions as and electrophile and Glu 211 oxidizes 2-PGA to form PEP. Lys 345 and Glu 211 work together to form a salt bridge that stabilizes Mg2+ ion. Lys 345 functions as general acid and Glu 211 forms a covalent intermediate.
Transcribed Image Text:Lys 345 functions as general base and Glu 211 functions as a general acid. Lys 345 functions as a electrophile and Glu 211 functions as a nucleophile. Glu 211 functions as and electrophile and Glu 211 oxidizes 2-PGA to form PEP. Lys 345 and Glu 211 work together to form a salt bridge that stabilizes Mg2+ ion. Lys 345 functions as general acid and Glu 211 forms a covalent intermediate.
Expert Solution
Step 1

Enolase is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. The enzyme displays a general acid-base mechanism of action. Lys345 and Glu211 of active site plays in important role in catalysis. Magnesium ions are an important co-factor for the enzyme.

steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS