Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
In lab 4, you calculated cholesterol levels of a sample directly based on the absorbance ratio of the sample to standard. What assumption are we making for this calculation to be accurate? O All the cholesterol in the sample is unesterified (.e. free cholesterol). There is a linear relationship between the cholesterol concentration of the sample and the absorbance of the sample. O The enzymatic reaction goes to completion, L.e. all substrates are converted to products. The HDL and LDL in the sample have different absorption maxima as compared to the cholesterol.

In lab 4, you calculated cholesterol levels of a sample directly based on the absorbance ratio of the sample to standard. What assumption are we making for this calculation to be accurate? O All the cholesterol in the sample is unesterified (.e. free cholesterol). There is a linear relationship between the cholesterol concentration of the sample and the absorbance of the sample. O The enzymatic reaction goes to completion, L.e. all substrates are converted to products. The HDL and LDL in the sample have different absorption maxima as compared to the cholesterol.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
In lab 4, you calculated cholesterol levels of a sample directly based on the absorbance ratio of the sample to standard. What assumption are we making for this calculation to be accurate? O All the cholesterol in the sample is unesterified (i.e. free cholesterol). There is a linear relationship between the cholesterol concentration of the sample and the absorbance of the sample. The enzymatic reaction goes to completion, i.e. all substrates are converted to products. The HDL and LDL in the sample have different absorption maxima as compared to the cholesterol.
Transcribed Image Text:In lab 4, you calculated cholesterol levels of a sample directly based on the absorbance ratio of the sample to standard. What assumption are we making for this calculation to be accurate? O All the cholesterol in the sample is unesterified (i.e. free cholesterol). There is a linear relationship between the cholesterol concentration of the sample and the absorbance of the sample. The enzymatic reaction goes to completion, i.e. all substrates are converted to products. The HDL and LDL in the sample have different absorption maxima as compared to the cholesterol.
Expert Solution
steps

Step by step

Solved in 4 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS