In E. coli, when glucose is present, galactose is used to make a component of the bacterial cell wall. When glucose is absent, galactose is MOSTLY used to make energy, but a little is used to make the cell wall. The enzymes for the conversion of galactose into a cell wall component are transcribed as an operon. The operon is transcribed at a high level when glucose is present and at a low level when glucose is absent. You isolate mutants that do not respond appropriately to the presence or absence of glucose, as summarized below. Galactose incorporation into cell wall strain + Glucose - Glucose Wild type High High High Low Mutant 1 High High Mutant 2 Mutant 3 Low Low Mutant 4 None None Mutant 5 High None Mutant 6 None None Which of the above mutants could have a mutation in the repressor for the above operon O Mutant 6 O Mutant 5 O Mutant 1
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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