In DNA sequencing by the Sanger method, O the template strand of the DNA is radioactive specific enzymes are used to cut the DNA into small pieces which are then separated by electrophoresis O dideoxy ATP (ddATP) is included in each of the four reaction mixtures before enzymatic synthesis of complementary strands the role of dideoxy CTP (ddCTP) is to occasionally terminate enzymatic synthesis of DNA where Gs occur in the template strands.

In DNA sequencing by the Sanger method, O the template strand of the DNA is radioactive specific enzymes are used to cut the DNA into small pieces which are then separated by electrophoresis O dideoxy ATP (ddATP) is included in each of the four reaction mixtures before enzymatic synthesis of complementary strands the role of dideoxy CTP (ddCTP) is to occasionally terminate enzymatic synthesis of DNA where Gs occur in the template strands.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter26: Synthesis And Degradation Of Nucleotides

Section: Chapter Questions

Problem 6P: Allosteric Regulation of Ribonucleotide Reductase by ATP and Deoxynucleotides Describe the...

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Several temperature-sensitive mutant strains of E. coli displaythe following characteristics. Predict what enzyme or function isbeing affected by each mutation. Okazaki fragments accumulate, and DNA synthesis is never completed
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