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In DNA sequencing by the Sanger method, O the template strand of the DNA is radioactive specific enzymes are used to cut the DNA into small pieces which are then separated by electrophoresis O dideoxy ATP (ddATP) is included in each of the four reaction mixtures before enzymatic synthesis of complementary strands the role of dideoxy CTP (ddCTP) is to occasionally terminate enzymatic synthesis of DNA where Gs occur in the template strands.

In DNA sequencing by the Sanger method, O the template strand of the DNA is radioactive specific enzymes are used to cut the DNA into small pieces which are then separated by electrophoresis O dideoxy ATP (ddATP) is included in each of the four reaction mixtures before enzymatic synthesis of complementary strands the role of dideoxy CTP (ddCTP) is to occasionally terminate enzymatic synthesis of DNA where Gs occur in the template strands.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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In DNA sequencing by the Sanger method, the template strand of the DNA is radioactive specific enzymes are used to cut the DNA into small pieces which are then separated by electrophoresis dideoxy ATP (ddATP) is included in each of the four reaction mixtures before enzymatic synthesis of complementary strands the role of dideoxy CTP (ddCTP) is to occasionally terminate enzymatic synthesis of DNA where Gs occur in the template strands.
Transcribed Image Text:In DNA sequencing by the Sanger method, the template strand of the DNA is radioactive specific enzymes are used to cut the DNA into small pieces which are then separated by electrophoresis dideoxy ATP (ddATP) is included in each of the four reaction mixtures before enzymatic synthesis of complementary strands the role of dideoxy CTP (ddCTP) is to occasionally terminate enzymatic synthesis of DNA where Gs occur in the template strands.
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