(4) blood cellsBase your answer to questions 4 through7 on the information below and on yourknowledge of biology.In 1973, Stanley Cohen and HerbertBoyer inserted a gene from an Africanclawed frog into a bacterium. Thebacterium then began producing aprotein directed by the code found on theinserted frog gene.4. The newly synthesized material in thebacterium is the direct result of(1) DNA replication(2) rapid DNA synthesis(3) the use of frog DNA(4) the use of bacterial DNA

Stanley Cohen and Herbert Boyer were the first introduce the genes from one living organism to…

Answered: In 1973, Stanley Cohen and Herbert…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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In the closed promoter complex Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a) the DNA is opened up a b b) the RNA polymerase has bound to the promoter c) the DNA polymerase has bound to the origin of replication d) none of the above
Which of the following typically causes chromosome mutations such as inversions and translocations? A) Base modifiers B) UV light C) ionizing radiation D) base analogues E) intercalating agents
Following are four processes common to most cloning experiments: a) transforming bacteria b) plating bacteria on selective medium c) cutting DNA with restriction endonucleases d) ligating DNA fragments Place components of this list in the order in which they would most likely occur during a cloning experiment.
Which of the following is NOT an example of a spontaneous mutation? A) errors in replication of DNA polymerase B) ALL of these are examples of spontaneous mutations C) covalent alteration of DNA by chemical products of fatty acid metabolism D) incorporation of a nucleotide analog during DNA synthesis E) failure to correct an apurinic site F) nondisjunction during meiosis
At the DNA level, a mutation in a protein coding region where a single base is replaced by another one. a) substitution O b) missense O c) nonsense O d) frameshift O e) B or C
In a process of production of a recombinant protein by E. coli cells, it was observed accumulation of acetate in the culture medium. In this situation, it can be said that: (a) certainly the process in question was being conducted in anaerobiosis (B).Acetate accumulation is advantageous for the process as the acetate formation reaction generates 1 molecule of ATP (c)Knowing that decreasing the temperature of the process causes a reduction in the rate of glycolysis, this could be a strategy to reduce the accumulation of acetate (d).the acetate formed can be re-assimilated by the cell if the glyoxylate pathway is activated at some point in the culture
The Drug AZT is used to treat AIDS, This drug is a _____ Inhibitor/Activator of _____ a) Competitive; Activator; DNA polymerase b) Noncompetitive; Inhibitor; DNA polymerase c) Competitive; Inhibitor; Reverse Transcriptase d) Noncompetitive; Activator; Reverse Transcriptase
A scientist isolates the DNA genome from a virus. She attempts to carry out a melting analysis but finds only 10% hypochromicity. (a) Suggest an explanation for the low value. (b) Why do you think she finds this much?
After several rounds of replication, if COVID19 RNA changes from 5’ GGGUACAUGGUAGCCCCCGUCGAG …. 3’ to 5’ GGGUACAUGGUAGCCCCCGUCGCCCCGUAG …. 3’ Which of the following describes the above event? a) an insertion mutation occurred b) a duplication mutation occurred c) a multi-points mutation occurred d) a point-series mutation occurred e) an inversion mutation occurred
Recombinant DNA molecule is produced by ligatinga) Two DNA segmentsb) Two m-RNA moleculesc) One m-RNA with one t-RNAd) One m-RNA with DNA segment
(a) Why the plasmid (containing the foreign DNA), together with the competentbacterial cells are heated at 42°C? b) What would be the next stage after heat shock? Discuss the process involved.c) How would you analyze the efficiency of the competent cells used duringtransformation?

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