If you wanted to test the effect of a 1:2000 dilution (2,000-fold) of lemon juice on PtK2 cells, how much of a 1:10 working stock of lemon juice would you add to the 6mls in an experimental flask?
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
. If you wanted to test the effect of a 1:2000 dilution (2,000-fold) of lemon juice on PtK2 cells, how much of a 1:10 working stock of lemon juice would you add to the 6mls in an experimental flask?
If you wanted to test the effect of a 1:500 dilution (500-fold) of coffee on PtK2 cells, how much of a 1:100 working stock of coffee would you add to 6mls in an experimental flask? Would this be a good volume to add? Why or why not?
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