If you are a medical technologist, which of the four methods of glucose determination would you employ? why? 2. Do you think PFF should be part of enzymatic method of glucose determination? Explain your answer. 3.Describe the accuracy and specificity of Nelson-Somogyi method.
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1. If you are a medical technologist, which of the four methods of glucose determination would you employ? why?
2. Do you think PFF should be part of enzymatic method of glucose determination? Explain your answer.
3.Describe the accuracy and specificity of Nelson-Somogyi method.
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Solved in 3 steps
- 1. Describe the accuracy and specificity of Nelson-Somogyi method 2. Do you think PFF should be part of enzymatic method of glucose determination? Explain your answer.1. Describe the role of sodium and phosphomolybdate reagent in Folin Wu method. 2. Describe the accuracy and specificity of Nelson-Somogyi method 3. Do you think PFF should be part of enzymatic method of glucose determination? Explain your answer.Q.No 2. Write a complete method for the measurement of the concentration of glucose by using spectrophotometer
- answer the following questioms.. 1.What is the action of invertase?2.What is the reagent used to detect glucose in the detection of invertase? What is its principle?1. What is the action of invertase? 2. What is the reagent used to detect glucose in the detection of invertase? What is its principle? 3. What is the reagent used to detect glucose in the oh and temperature experiments? What is its principle? 4. What is the optimum temperature for invertase reactions? 5. What is the optimum pH for the invertase reactions? 6. What is the wavelength used for the spectrophotometer? What is the principle of the spectrophotometer?a. From experimental data obtained in Figure 1, construct a plot and equation that determines the rate constant, kM, of the MOX activity in conditions A and B of growth culture of H. polymorpha on methanol. Justify the selection of plot and findings. b. From the data behaviour presented in Figure 1, describe the enzymatic activity of MOX. What factors may influence the enzymatic activity of MOX at different condition batch cultures of H. polymorpha.
- Urine samples for each patient were tested for ketone bodies, and a Benedict's reagent and glucose oxidase reaction was also completed. The results are given in the table below, with - indicating a negative result and +, ++ and +++ indicating a positive result of different intensity. Patient A Patient B Patient C Patient D Patient E Ketone Bodies +++ Benedict's Reagent +++ - +++ - +++ Glucose Oxidase +++ +++ + 4. For each individual patient A-E, using the WHO guidelines for the diagnosis of diabetes, write a short paragraph to explain: (a) what you can deduce from the fasting blood glucose and the 2 hour sample of that patient1. Comparative characteristics of bioenergetics of aerobic and anaerobic glucose oxidation, Pasteur effect. 2. The role of Ca2+ in muscle contraction. 3. The patient blood test after a course of atherosclerosis treatment showed an increasing a level of antiatherogenic fraction of lipoproteins. The increase of what lipoprotein may confirm the effectiveness of treatment1.Which test is used to differentiate between fermentative and oxidative metabolism?describe the different results that may be observed with this test
- 0.0025 0.002 0.0015 0.001 0.0005 10 20 30 40 50 60 70 80 -0.0005 Temperature (C) 1.What trend did you observe for the rate of peroxidase compared to the temperature of the enzyme? 2.What was the optimum temperature tested for the enzyme peroxidase? 3.Did any of the temperatures tested cause the enzyme to denature? Which one? Rate (abs/sec @ 500nm)1. Describe the specificity of glucose oxidase. Compare it with the three other methods.1. Which water-soluble vitamin can regenerate vitamin E after it has been oxidized? 2. Which of the following has been associated with an increased risk for cancer? 3. Design a lunch or dinner that would be suitable for a college student following the DASH diet and providing no more than 650 kcal. 4. Discuss the various roles and relationships between antioxidant nutrients and phytochemicals and cancer. Include a discussion of the pros and cons of antioxidant supplementation versus acquiring antioxidants through foods. 5. Vitamin E is critical for E? 6. Which of the following foods are the richest sources of vitamin E 7. Diseases that cause the malabsorption of fat can result in a deficiency of