i) Write down the equation derived from your Excel generated standard curve and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision iii) state the answer in a full sentence. (note: avoid mathematical symbols [=, +, -, etc] in your answer sentence).
i) Write down the equation derived from your Excel generated standard curve and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision iii) state the answer in a full sentence. (note: avoid mathematical symbols [=, +, -, etc] in your answer sentence).
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
i) Write down the equation derived from your Excel generated standard curve and describe its components;
ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision
iii) state the answer in a full sentence. (note: avoid mathematical symbols [=, +, -, etc] in your answer sentence).
Transcribed Image Text:Sample
Blank
Standard
1
Standard
2
Standard
3
Standard
4
Glycine
unknown
#
Conc.
(mm)
0
0.02
0.04
0.06
0.08
1
A570 (1) A570 (2) A570 (3)
0
0
0.125 0.115
O
0-112
Average
A570
O
0.1173
0-178
0.198 0.167
0-178 0.295 0-304
0.452 0.451 0.414
0.439
0.394 0.330 0.411 0.3783
0-181
0.259
Transcribed Image Text:Concentration (Glycine mM
0.5
0.45
0.4
0.35
0.3
0.25
0.2
0.15
0.1
0.05
0
-0.05 0
0.1
0.2
Absorbance of Glycine
y = 0.5099Glycine Concentration - 0.0047
R² = 0.9646
0.3
0.4
0.5
Absorbance (570mM)
0.6
0.7
0.8
0.9
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