I need The brief explanation and the predicted results for each organism in the list. Pre-Lab Assignment Title: Isolation Technique streak Plates*Due for each new lab - at the start of lab and before the lab/safety introduction. Use a new sheet for each different procedure/test.Pre-lab must be completed PRIOR to entrance to the lab. This is your ticket into the lab room. No exceptions will be made.Purpose:Procedure:List the steps in theprocedure.Cultures needed:*Use proper namingand shorthandnotationsChanges toProtocol:Results:*Describe what apositive/negativeresult will look likePredicted Results/Other:List predicted resultsfor each organismLearn how to isolate mixtures of bacteria into pure cultureand to recognize the specific colony morphology of some bacte-ria.1-Obtain and label the nutrient agar plates needed for theStreak plates, we need one for each pure culture and oneFor each mixed culture.2-Using aseptic techniques as demonstrated by the instructor,make streak plates of all the cultures. Remember that youshould not use too large an ino culum and that you should useas much of the surface of the medium as possible. Avoiddigging into the medium. If we make a mistake, repeat thestreak plate with a fresh plate, if available..3-Place all plates in a 37°c incubator in an inverted position(why?). Allow them to incubate for 24 to 48 hours.4-Following incubation of the cultures, record your observations.Write a description of each colony type and try to identify eachorganism in the mixed culture plate!5-If you did not get isolated colonies, examine your Streak Forpossible reasons why. Repeat the experiment untilobtained isolated colonies if instructed.youhave6- Record the observations on the Laboratory Report Form.Twenty-Four-hour mixed both cultures of Bacillus subtilisStaphylococcus epidermidis, Pseudomonas aeruginosa,serratia marcescens, (SE) (SM) (PA) (BS) (Mix)no slantsPositive Result:Obtain isolate colonies.Negative Result:Not obtain isolate colonies.Brief Explanation:Brief Explanation:

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Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

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I need The brief explanation and the predicted results for each organism in the list.

Pre-Lab Assignment Title: Isolation Technique streak Plates
*Due for each new lab - at the start of lab and before the lab/safety introduction. Use a new sheet for each different procedure/test.
Pre-lab must be completed PRIOR to entrance to the lab. This is your ticket into the lab room. No exceptions will be made.
Purpose:
Procedure:
List the steps in the
procedure.
Cultures needed:
*Use proper naming
and shorthand
notations
Changes to
Protocol:
Results:
*Describe what a
positive/negative
result will look like
Predicted Results/
Other:
List predicted results
for each organism
Learn how to isolate mixtures of bacteria into pure culture
and to recognize the specific colony morphology of some bacte-
ria.
1-Obtain and label the nutrient agar plates needed for the
Streak plates, we need one for each pure culture and one
For each mixed culture.
2-Using aseptic techniques as demonstrated by the instructor,
make streak plates of all the cultures. Remember that you
should not use too large an ino culum and that you should use
as much of the surface of the medium as possible. Avoid
digging into the medium. If we make a mistake, repeat the
streak plate with a fresh plate, if available..
3-Place all plates in a 37°c incubator in an inverted position
(why?). Allow them to incubate for 24 to 48 hours.
4-Following incubation of the cultures, record your observations.
Write a description of each colony type and try to identify each
organism in the mixed culture plate!
5-If you did not get isolated colonies, examine your Streak For
possible reasons why. Repeat the experiment until
obtained isolated colonies if instructed.
you
have
6- Record the observations on the Laboratory Report Form.
Twenty-Four-hour mixed both cultures of Bacillus subtilis
Staphylococcus epidermidis, Pseudomonas aeruginosa,
serratia marcescens, (SE) (SM) (PA) (BS) (Mix)
no slants
Positive Result:
Obtain isolate colonies.
Negative Result:
Not obtain isolate colonies.
Brief Explanation:
Brief Explanation:

Expert Solution

Step 1

This lab will introduce the technique of streak plate isolation, which is a method used to isolate a single bacterial colony from a culture. This technique is used to identify a particular bacteria and can be used to determine the type of bacteria present in a sample. The lab will involve obtaining a sterile inoculating loop, streak plate, and a culture of bacteria. The loop will be used to draw lines on the surface of the streak plate which will be used to spread the bacteria. The plate will then be incubated for 24-48 hours at a temperature that is appropriate for the bacteria before observing for bacterial growth and isolating a single colony.

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