3. (a) Comparing the culture-dependant (e.g., colony forming units count) and DNA-based (e.g., PCR) bacterial number quantification methods, what are the primarydisadvantages of using culture-dependent methods to quantify bacterial numbers in anenvironmental sample? Use no more than three sentences or bullet points to presentyour answer succinctly. (b) At a local wastewater treatment plant receiving municipal wastewater, thesecondary treatment units have been performing severely under the designedbenchmarks, resulting in poor effluent quality. You, a wastewater treatment engineer,suspected that the wastewater treatment microbes in the units might be inhibited.What factors do you want to check to find out potential causalities of the inhibition?List the most common four factors that you want to start your investigation with.e(ii)(iii)_(iv).

Wastewater treatment is the process of removing contaminants and pollutants from wastewater to make…

Answered: 3. (a) Comparing the culture-dependant…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

1.What is a physiologic characteristic common among majority of cocci that relates to their grouping?2.what evidence can you give to support this physiologic activity?3.What is the significance of the characteristics grouping of cocci?4. Why do bacilli show less tendency to form groups?5. Can bacilli apper coccoid right after division? Support your answer. 6. Give 5 difference between rigid from flexible spirilla 7. To what structure do you attribute the flexibility of the spirochete famili?
(a) Describe the actions with reason that should be taken under the following situations: (i) An unlabelled collection tube with a requisition for a V Leiden test is received in the laboratory. (ii) After PCR, the amplification control has failed to yield a product. (iii) DNA is stored overnight in a refrigerator set at 8oC but reads 14 oC. (iv) An isolated DNA sample is to be stored for at least 6 months. (v) The expiration date on a reagent has passed. (b) Explain briefly about genetic testing for new-born, including the importance of genetic testing and the disorder that genetic testing can detect.
10) With the disk test, what specifically are you looking for to know if a bacteria is susceptible for an antibiotic? 11) What exactly does bacitracin do to bacteria to cause them to die or stop dividing?
Given the illustration and values below, determine the concentration of the original sample. Report results in CFU/mL or colony-forming unit/milliliter. Note that in order to observe the accuracy of results, culture plates with countable colonies between 25-250 CFU are considered in standard bacterial plate count.
A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.90% NaCl. Three consecutive dilutions were further made by using I ml aliquot in 9 ml of 0.90% NaCl. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. 田 Sampling COUNTS 1st dilution 54; 61 2nd dilution 3rd dilution 4h dilution 1st 3; 7 0; 0 0,0 2nd TNTC TNCT 242: 233 28: 37 3rd TNTC TNTC INTC 249-246 * TNTC = Too numerous to count i. Illustrate the dilution series used and label the final dilution of each dilution. ii. Determine the bacterial count (CFU/ml) every 4 hours of incubation for 12 hours. Show all computations.
Could you please assist with correct number?
In the first step of your phage therapy design process, you isolate the exact Mycobacterium strain from the patient. What is the most important thing must you establish next about this bacterial isolate?
1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results. 3. Please provide the scientific name of your microbe that was used in the UV experiment (i.e. S. aureus). Compare your plates and interpret/analyze your results. Please discuss your findings and any patterns you were able to gather. 4. After performing the “Effects of Antiseptics & Disinfectants” lab which agent(s) showed potential to control S. marcescens growth? P. aeruginosa? Please explain why you believe these agent(s) work. 5. What purpose does water serve in the “Effects of Antiseptics & Disinfectants” lab? What did you…
You are tasked to isolate a certain species of bacteria from a mixed culture to further characterize and identify the strain. Which technique/s is Best to perform? How shall the procedure be carried out? Explain.
If you were to analyze three separate colonies from the plate above, would you expect them all to be the same species of bacteria? Why or why not?
1.What colony characterisitics can be used for differentiation of bacterial species? As an example, compare the properties of colonies of Serratia marcescens and Micrococcus luteus on your streak plate. 2.Define the term colony as it relates to bacterial growth on solid media? 3. why is dilution a necessary part of pure culture preperation? 4.What advantage(s) does the streak-plate method have over the pour-plate method?
You perform the Methyl-Red(MR)& Voges-Proskauer(VP)test and gather the followingresults: (Uploaded picture). VP test result is on the left, MR test result is on the right. A) Please interpret these results. B) Your fellow researcher and yourself are sure your bacterial sample is either E. coli or P. vulargis. Basedupon the work we have done in lab which methods will help you determine which bacteria it is? Please explain why, the results you would want to observe and provide analysis of those results.

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