I am trying to figure out these four things. What is the independent variable, what is the dependent variable, what are the controlled variables and why this experiment doesn’t use a control.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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I am trying to figure out these four things. What is the independent variable, what is the dependent variable, what are the controlled variables and why this experiment doesn’t use a control.
**Text for Educational Website:**

As you know from your experiments with the enzyme amylase, starch reacts with iodine to form a bluish/purple/blackish/purple color. In leaves that are actively photosynthesizing, the glucose produced via photosynthesis is immediately converted into starch for storage. If leaves are placed in a near-boiling alcohol bath, the alcohol will dissolve and extract the pigments, leaving the starch behind. Leaves treated in this way become a light beige color and are largely translucent. Because iodine reacts with starch to produce a darker color, the iodine will show where starch is present, and therefore where photosynthesis occurred in the cleared leaf with iodide. By comparing the location of starch in the leaf to the location of different pigments, you can deduce which pigments are photosynthetically active. 

The question you are addressing is: Which pigments are most active in photosynthesis? 

**Alternative Hypotheses:**

In this experiment, there are several alternative hypotheses. They are as follows:

- H1: Chlorophylls are the most photo-synthetically active pigments.
- H2: Xanthophylls are the most photosynthetically active pigments.
- H3: Carotenes are the most photo-synthetically active pigments.
- H4: Anthocyanins are the most photo-synthetically active pigments.
Transcribed Image Text:**Text for Educational Website:** As you know from your experiments with the enzyme amylase, starch reacts with iodine to form a bluish/purple/blackish/purple color. In leaves that are actively photosynthesizing, the glucose produced via photosynthesis is immediately converted into starch for storage. If leaves are placed in a near-boiling alcohol bath, the alcohol will dissolve and extract the pigments, leaving the starch behind. Leaves treated in this way become a light beige color and are largely translucent. Because iodine reacts with starch to produce a darker color, the iodine will show where starch is present, and therefore where photosynthesis occurred in the cleared leaf with iodide. By comparing the location of starch in the leaf to the location of different pigments, you can deduce which pigments are photosynthetically active. The question you are addressing is: Which pigments are most active in photosynthesis? **Alternative Hypotheses:** In this experiment, there are several alternative hypotheses. They are as follows: - H1: Chlorophylls are the most photo-synthetically active pigments. - H2: Xanthophylls are the most photosynthetically active pigments. - H3: Carotenes are the most photo-synthetically active pigments. - H4: Anthocyanins are the most photo-synthetically active pigments.
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