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How did they get answer. Why did they do 2n?

How did they get answer. Why did they do 2n?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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How did they get answer. Why did they do 2n?
2) A scientist wants to increase the amount of sample of a DNA sequence of 100 bps. Calculate the number cycles of PCR required to generate 1 ng of DNA, when starting from a single copy. Note that the molar mass of an average DNA bp is 600 g/mol.
Transcribed Image Text:2) A scientist wants to increase the amount of sample of a DNA sequence of 100 bps. Calculate the number cycles of PCR required to generate 1 ng of DNA, when starting from a single copy. Note that the molar mass of an average DNA bp is 600 g/mol.
or copies in "n" PCR cycles 2n = DNA molecules We need to solve the equation for n. Mol=mass/mr=1*10-⁹g / 100*600g/mol = 1.6*10-14 moles, which is 1010 molecules (Avogadro number: 6*10²³) 2n= 1010 log2n=log1010, n*log2=10*log10 ➜ n=10*log10/log2 = 33.2 → 34 cycles
Transcribed Image Text:or copies in "n" PCR cycles 2n = DNA molecules We need to solve the equation for n. Mol=mass/mr=1*10-⁹g / 100*600g/mol = 1.6*10-14 moles, which is 1010 molecules (Avogadro number: 6*10²³) 2n= 1010 log2n=log1010, n*log2=10*log10 ➜ n=10*log10/log2 = 33.2 → 34 cycles
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