mulation Activity ctivity, you will be provided with the DNA nucleotide ce that codes for a hypothetical protein. The code will ided to you in three fragments. You will have to tran- he code into mRNA, remove an intron segment, and e the mRNA into the protein. In addition, you will identify the beginning fragment, the middle fragment, end fragment. dure Opy each of the following quences onto a separate ece of paper. TCTTCCCTCCTAAACGTTCAACCGGTTCTTAATCCGC CGCCAGGGCCCCGCCCCTCAGAAGTTGGT Sequence B TCAGACGTTTTTGCCCCGTAACAACTTGTTACAA CATGGTCATAAACGTCAGAGATGGTCAATCTCTTAAT GACT Sequence C TACAAACATGTAAACACACCCTCAGTGGACCAACTC CGCA ACATAAACCAAACACCGCTCGCGCCGAAAAA GATATGG 2. Divide the sequences into triplets (codons) by putting a slash between each group of three bases. TIVITY 5.4.1 continued anscribe the DNA into mRNA. entify the middle, end, and beginning sequence. Use ur knowledge of start and stop codons to help you gure it out. move codons 24 to 66, including codon 66. anslate the mRNA into protein using the genetic de. sis hich fragment was the beginning fragment? How you know? hich fragment was the end fragment? How do you now? c) Codons 24 to 66 represent an intron. At what point in the process of protein synthesis are introns removed? What is the name of the enzyme responsible for this excision? (d) How many amino acids does this protein contain? (e) Is this genetic sequence eukaryotic or prokaryotic? How do you know? (f) If you worked backward, starting with the amino acid sequence of the protein, would you obtain the same DNA nucleotide sequence? Why or why not? (g) Provide the anticodon sequence that would build this protein.
mulation Activity ctivity, you will be provided with the DNA nucleotide ce that codes for a hypothetical protein. The code will ided to you in three fragments. You will have to tran- he code into mRNA, remove an intron segment, and e the mRNA into the protein. In addition, you will identify the beginning fragment, the middle fragment, end fragment. dure Opy each of the following quences onto a separate ece of paper. TCTTCCCTCCTAAACGTTCAACCGGTTCTTAATCCGC CGCCAGGGCCCCGCCCCTCAGAAGTTGGT Sequence B TCAGACGTTTTTGCCCCGTAACAACTTGTTACAA CATGGTCATAAACGTCAGAGATGGTCAATCTCTTAAT GACT Sequence C TACAAACATGTAAACACACCCTCAGTGGACCAACTC CGCA ACATAAACCAAACACCGCTCGCGCCGAAAAA GATATGG 2. Divide the sequences into triplets (codons) by putting a slash between each group of three bases. TIVITY 5.4.1 continued anscribe the DNA into mRNA. entify the middle, end, and beginning sequence. Use ur knowledge of start and stop codons to help you gure it out. move codons 24 to 66, including codon 66. anslate the mRNA into protein using the genetic de. sis hich fragment was the beginning fragment? How you know? hich fragment was the end fragment? How do you now? c) Codons 24 to 66 represent an intron. At what point in the process of protein synthesis are introns removed? What is the name of the enzyme responsible for this excision? (d) How many amino acids does this protein contain? (e) Is this genetic sequence eukaryotic or prokaryotic? How do you know? (f) If you worked backward, starting with the amino acid sequence of the protein, would you obtain the same DNA nucleotide sequence? Why or why not? (g) Provide the anticodon sequence that would build this protein.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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Transcribed Image Text:ACTIVITY 5.4.1
Synthesis of a Protein:
A Simulation Activity
In this activity, you will be provided with the DNA nucleotide
sequence that codes for a hypothetical protein. The code will
be provided to you in three fragments. You will have to tran-
scribe the code into mRNA, remove an intron segment, and
translate the mRNA into the protein. In addition, you will
have to identify the beginning fragment, the middle fragment,
and the end fragment.
Procedure
1. Copy each of the following quences onto a separate
piece of paper.
Sequence A
TCTTCCCTCCTAAACGTTCAACCGGTTCTTAATCCGC
CGCCAGGGCCCCGCCCCTCAGAAGTTGGT
Sequence B
TCAGACGTTTTTGCCCCGTAACAACTTGTTACAA
CATGGTCATAAACGTCAGAGATGGTCAATCTCTTAAT
GACT
Sequence C
TACAAACATGTAAACACACCCTCAGTGGACCAACTC
CGCA ACATAAACCAAACACCGCTCGCGCCGAAAAA
GATATGG
2. Divide the sequences into triplets (codons) by putting
a slash between each group of three bases.
ACTIVITY 5.4.1 continued
3. Transcribe the DNA into mRNA.
4. Identify the middle, end, and beginning sequence. Use
your knowledge of start and stop codons to help you
figure it out.
5. Remove codons 24 to 66, including codon 66.
6. Translate the mRNA into protein using the genetic
code.
Analysis
(a) Which fragment was the beginning fragment? How
do you know?
>) Which fragment was the end fragment? How do you
know?
c) Codons 24 to 66 represent an intron. At what point in
the process of protein synthesis are introns removed?
What is the name of the enzyme responsible for this
excision?
(d) How many amino acids does this protein contain?
(e) Is this genetic sequence eukaryotic or prokaryotic?
How do you know?
(f) If you worked backward, starting with the amino acid
sequence of the protein, would you obtain the same
DNA nucleotide sequence? Why or why not?
(g) Provide the anticodon sequence that would build this
protein.
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