How can you calculate the concentration of a solution with UV-vis spectroscopy if you do NOT know the molar absorption coefficient? (a) Add blue dye to the sample and then subtract the blank sample's absorbance. (b) Use Beer's Law by assigning the molar absorption coefficient a value of 1 (e = 1). (c) Create a calibration curve which is a plot of absorbance vs concentration. (d) Calculate the rate constant. (e) Divide the measured absorbance by the product of l´e.

How can you calculate the concentration of a solution with UV-vis spectroscopy if you do NOT know the molar absorption coefficient? (a) Add blue dye to the sample and then subtract the blank sample's absorbance. (b) Use Beer's Law by assigning the molar absorption coefficient a value of 1 (e = 1). (c) Create a calibration curve which is a plot of absorbance vs concentration. (d) Calculate the rate constant. (e) Divide the measured absorbance by the product of l´e.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

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