Given the chromatogram below in a normal phase, which samples would be the most polar? A O Samples C and D O Samples Band X O Samples B and C O Samples A and D
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- Given the chromatogram below in a normal phase, which samples would be the least polar? O Samples Band C O Samples C and D O Samples A and D O Samples B and XGiven the chromatogram below, which of the following is the possible identity of unknown sample Y? B. O Sample E O Sample F O Sample Z O Sample B ONIn a reverse phase chromatography set up, the component that yields the lowest Rf value is likely to be O Both polarity O Polar O Non-polar O Cannot be determined
- For the same chromatographic column and experimental conditions Rs when H = 3 cm = Rs when H = 5 cm Rs when H = 3 cm > Rs when H = 5 cm Rs when H = 3 cm < Rs when H = 5 cm The two quantities cannot be compared due to insufficient information.Your pharmacy has on hand Tetracycline 250 mg/5 mL liquid.You receive a prescription for Tetracycline syrup 500 mg qid for 7 days. How many total milliliters would you dispense? Your pharmacy has on hand Prednisone 5 mg tablets.You receive a prescription for Prednisone 5 mg ii tabs stat and qid x 1 day, then ii tabs tid x 2 days, then ii tabs bid x 2 days, then i tab tid x 3 days, then i tab bid x 3days, then i tab qd x 4 days How many tablets would you dispense for this prescription? How many days will the patient take this medication for? Your pharmacy has on hand Dilantin 100mg capsules.You receive a prescription for Dilantin 100 mg 1 cap po in the AM & PM, 200mg (2 caps) po at noon, and 300mg (3 caps) po at hs. Dispense a 30 day supply. (using the 100mg capsules you have in stock) How many capsules will you dispense? Your pharmacy has on hand Dakins Irrigation Solution 1 liter.You receive a prescription for Dakins Irrigation Solution Irrigate chest tube with 100 mL…Four amino acid samples were subjected to a reverse-phase chromatography set up and yielded the following Rf values. Which among the samples is the most polar? Sample Rf values A 0,49 0.53 0.21 0.55 OD OB
- Which of the following scientists use this X-ray crystallography?Four amino acid samples were subjected to a reverse-phase chromatography set up and yielded the following, Rf values. Which among the samples is the most polar? Sample Rf values A 0.49 0.53 0.21 0.55 O A B.What happens if you exclude one or more data sets of the peak area ratio of the chromatograph, how does it affect the mean concentration of the analyte and its linear requation
- Figure 3: ● ● ● ● ● ● KDa ● 97.4 66.2 45.0 ● 31.0- 21.5 14.4 S-1 p-1 S-2 2-0 This figure was generated by centrifuging a pura sample of protein, removing the supernatant, and resuspending the pellet in the same volume as the supernatant to allow direct comparison. The supernatant and pellet samples were then prepared for SDS-PAGE identically and run via normal SDS-PAGE procedures. In the figure, "s" means supernatant and "p" means pellet. The text or number after the dash represents a different condition. For example, s-1 and p-1 are the supernatant and pellet samples under condition 1. It is not shown, but under wild-type conditions, essentially all of the protein is found in the supernatant. S-3 ● What does the intensity of each band represent? ● Would you find soluble protein in the supernatant or pellet? Why? Would you find aggregated protein in the supernatant or pellet? Why? For each condition (there are 5 different conditions), is there a higher percentage of the total protein…A 1:1000 dilution of 1mM NaCl would give: 100µM 1µM 1nM 10µM 1M 100mM 1mM 100nM 10mM(a) You decide to purify the 5-HT 2A receptor. The protein has a predicted molecular Mass of 46.54 kDa and isoelectric point of 8.94. Will the 5-HT2A Receptor protein Bind to an anion exchange column if you are using a buffer containing 150m M NaCl, 50 mM carbonate buffer, pH6.0, and 10% glycerol? (b) After performing affinity chromatography purification, you run a SDS page gel showing The following: 250 150 100 75 50 37 25 . 15 10 Standards Sample | || | Explain why you see four bands in the sample lane? Label the band that corresponds to Your protein of interest. What do you think is going on here?
