For the competitive, noncompetitive and uncompetitive inhibition type investigate following figures and determine relationship between inhibitor concentration and Vmax and Km for each mechanism.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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For the competitive, noncompetitive and uncompetitive inhibition type investigate following figures and determine relationship between inhibitor concentration and Vmax and Km for each mechanism.

1
1
1
V.
(a) Competitive inhibition
[S]
max
E + S
ES
E + P
[I]
α3
I
a = 2
a = 1
EI
No inhibitor
1
aKm
Slope
max
max
1
1
Competitive inhibition
[S] \mM
1
1
a'
+
V.
(b) Uncompetitive inhibition
V.
max
max
E + S =
ES
E + P
a' = 2
[I]
I
a'=1.5
a'=1
ESI
1
Km
1
1
Uncompetitive inhibition.
[S]\mM
1
Vo
(aKm) 1
+
[S]
a'
(c) Mixed inhibition
Vmax
max
E + S
ES
E + P
+
I
I
[I]
K,
K{
EI + S
ESI
No inhibitor
S.
1
[S] (mM
Mixed inhibition.
µM/min)
Transcribed Image Text:1 1 1 V. (a) Competitive inhibition [S] max E + S ES E + P [I] α3 I a = 2 a = 1 EI No inhibitor 1 aKm Slope max max 1 1 Competitive inhibition [S] \mM 1 1 a' + V. (b) Uncompetitive inhibition V. max max E + S = ES E + P a' = 2 [I] I a'=1.5 a'=1 ESI 1 Km 1 1 Uncompetitive inhibition. [S]\mM 1 Vo (aKm) 1 + [S] a' (c) Mixed inhibition Vmax max E + S ES E + P + I I [I] K, K{ EI + S ESI No inhibitor S. 1 [S] (mM Mixed inhibition. µM/min)
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