For the competitive, noncompetitive and uncompetitive inhibition type investigate following figures and determine relationship between inhibitor concentration and Vmax and Km for each mechanism.
For the competitive, noncompetitive and uncompetitive inhibition type investigate following figures and determine relationship between inhibitor concentration and Vmax and Km for each mechanism.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
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For the competitive, noncompetitive and uncompetitive inhibition type investigate following figures and determine relationship between inhibitor concentration and Vmax and Km for each mechanism.
![1
1
1
V.
(a) Competitive inhibition
[S]
max
E + S
ES
E + P
[I]
α3
I
a = 2
a = 1
EI
No inhibitor
1
aKm
Slope
max
max
1
1
Competitive inhibition
[S] \mM
1
1
a'
+
V.
(b) Uncompetitive inhibition
V.
max
max
E + S =
ES
E + P
a' = 2
[I]
I
a'=1.5
a'=1
ESI
1
Km
1
1
Uncompetitive inhibition.
[S]\mM
1
Vo
(aKm) 1
+
[S]
a'
(c) Mixed inhibition
Vmax
max
E + S
ES
E + P
+
I
I
[I]
K,
K{
EI + S
ESI
No inhibitor
S.
1
[S] (mM
Mixed inhibition.
µM/min)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F0d50289a-9a35-4f98-8e42-2a2594983e7b%2F1dea016f-ed9b-4239-93b3-e6094d2dbfb9%2F5k7as19_processed.png&w=3840&q=75)
Transcribed Image Text:1
1
1
V.
(a) Competitive inhibition
[S]
max
E + S
ES
E + P
[I]
α3
I
a = 2
a = 1
EI
No inhibitor
1
aKm
Slope
max
max
1
1
Competitive inhibition
[S] \mM
1
1
a'
+
V.
(b) Uncompetitive inhibition
V.
max
max
E + S =
ES
E + P
a' = 2
[I]
I
a'=1.5
a'=1
ESI
1
Km
1
1
Uncompetitive inhibition.
[S]\mM
1
Vo
(aKm) 1
+
[S]
a'
(c) Mixed inhibition
Vmax
max
E + S
ES
E + P
+
I
I
[I]
K,
K{
EI + S
ESI
No inhibitor
S.
1
[S] (mM
Mixed inhibition.
µM/min)
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