For the beta-galactosidase assay, as controls, you plate bacteria known to be able to digest lactose (Lac+) and bacteria known to be unable to digest lactose (Lac-) on LB + X-Gal plates. For the beta-galactosidase assay, as controls, you plate bacteria known to be able to digest lactose(Lac+) and bacteria known to be unable to digest lactose (Lac-) on LB + X-Gal plates.After an overnight incubation at 37C you expect to see growth with blue colonieson the platewith the Lac+ bacteria and no growth (no colonies)for the plate with the Lac- bacteria.Note: The bacteria used in these assays normally appear whiteish yellow. X-Gal is the substrate forthe enzyme beta-galactosidase and will turn blue when digested by the enzyme.

Name: For the beta-galactosidase assay, as controls, you plate bacteria know
Uploaded: 2024-03-20T06:42:41.000Z
Channel: Bartleby
Description: For the beta-galactosidase assay, as controls, you plate bacteria known to be able to digest lactose (Lac+) and bacteria known to be unable to digest lactose (Lac-) on LB + X-Gal plates. For the beta-galactosidase assay, as controls, you plate bacter

the above experiment is about the molecular screening process to check the gene transformationDuring the cloning process, when we clone our gene of interest into a vector, now to check whether the gene is successfully transformed into a bacteria with our desired gene of interest. now we plate bacteria which is known to be able to digest lactose Lac+ and bacteria known to be unable to digest lactose (lac-) on LB + X-Gal plates. as you know we insert a gene into multiple cloning sites now if the gene is inserted then there will be no intact gene so no beta galactosidase activity so it can't use x gal as substrate so you will get a white colony. and that means transformation is successful. now if the gene is not inserted that means somehow vector get ligated then we got false positive colony and in them, there is no gene of interest that means the MCS multiple cloning site areas is intact so the gene is functional so the beta-galactosidase will work and use x-gal as a substrate and give blue colony and it means there is no transformation. and in the above in one column it says no colony i.e for control that specifies that there is no contamination.