For protein X, you do not know the extinction coefficient of the substrate to calculate the true concentration. Therefore you are planning to calculate the initial rate of reaction as a change in absorbance per unit of time. After you did the experiment, you found that the the straightest portion of your graph is between 0 minute and 1 minute, and the absorbance changes from 0.865 to 0.132 within one minute. Calculate the initial velocity, which will be expressed as min-1.

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Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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For protein X, you do not know the extinction coefficient of the substrate to calculate the true
concentration. Therefore you are planning to calculate the initial rate of reaction as a change in absorbance per
unit of time. After you did the experiment, you found that the the straightest portion of your graph is between 0
minute and 1 minute, and the absorbance changes from 0.865 to 0.132 within one minute. Calculate the initial
velocity, which will be expressed as min-1.
Transcribed Image Text:For protein X, you do not know the extinction coefficient of the substrate to calculate the true concentration. Therefore you are planning to calculate the initial rate of reaction as a change in absorbance per unit of time. After you did the experiment, you found that the the straightest portion of your graph is between 0 minute and 1 minute, and the absorbance changes from 0.865 to 0.132 within one minute. Calculate the initial velocity, which will be expressed as min-1.
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