For protein X, you do not know the extinction coefficient of the substrate to calculate the true concentration. Therefore you are planning to calculate the initial rate of reaction as a change in absorbance per unit of time. After you did the experiment, you found that the the straightest portion of your graph is between 0 minute and 1 minute, and the absorbance changes from 0.865 to 0.132 within one minute. Calculate the initial velocity, which will be expressed as min-1.

For protein X, you do not know the extinction coefficient of the substrate to calculate the true concentration. Therefore you are planning to calculate the initial rate of reaction as a change in absorbance per unit of time. After you did the experiment, you found that the the straightest portion of your graph is between 0 minute and 1 minute, and the absorbance changes from 0.865 to 0.132 within one minute. Calculate the initial velocity, which will be expressed as min-1.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

For protein X, you do not know the extinction coefficient of the substrate to calculate the true
concentration. Therefore you are planning to calculate the initial rate of reaction as a change in absorbance per
unit of time. After you did the experiment, you found that the the straightest portion of your graph is between 0
minute and 1 minute, and the absorbance changes from 0.865 to 0.132 within one minute. Calculate the initial
velocity, which will be expressed as min-1.

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