Explain why these two DNA samples give different results, when they're both 50% G-C. Fraction of DNA 1 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 ---E coli DNA -Potoroo DNA
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Gilbert's potoroo (Potorous tridactylus, also known as the long-nosed potoroo) is a small marsupial mammal found in Australia; it is considered to be the world's most endangered marsupial. Like most animals, its genome is ~50% G-C pairs. DNA from E. coli, a species of bacteria, is also ~50% G-C. If you randomly shear E. coli DNA into 1000 bp fragments and put it through density gradient equilibrium centrifugation, you will find that all the DNA bands at the same place in the gradient, and if you graph the distribution of DNA fragments in the gradient you will get a single peak (see below). If you perform the same experiment with potoroo DNA, you will find that a small fraction of the DNA fragments band separately in the gradient (at a different density) and give rise to a small "satellite" peak on a graph of the distribution of DNA fragments in the gradient (see below).
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