Q: How real time RT-PCR can be used in detecting SARS-nCov2 virus? Please explain at your own words.
A: Reverse transcription- Polymerase chain reaction (RT-PCR) is a technique in which the RNA is…
Q: Discuss the recombinant DNA techniques that could be used to develop detection systems for the…
A: Deoxyribonucleic acid (DNA) is a molecule made up of two polynucleotide chains that coil around each…
Q: Differentiate Invitrogen pCR® II-TOPO® Vector from pBR322 Vector.
A: Molecular cloning : Production of genetically identical copies of a cell or its genetic material is…
Q: In the extraordinary times in which we live, biotechnology is playing a vital role in helping to…
A: COVID-19 affects different people in different ways- Most common symptoms: fever dry cough…
Q: During the adenoviral SARS-CoV2 vaccine development process, the plasmid encoding the Spike protein…
A: Plasmid is a circular double stranded DNA molecule found in bacteria and is capable of self…
Q: You are counting plaques on your plaque assay plates made from serial dilutions of your high titer…
A: A serial dilution is any dilution in which the concentration decreases by the same dilutions in each…
Q: The SARS-CoV-2 genome is a polycistronic genome. What does the term polycistronic refer to
A: The covid pandemic as we all know is the disease associated with virus SARS-CoV-2.
Q: prc nt'
A: Scientists use the PCR (polymerase chain reaction) technology to amplify small amounts of RNA from…
Q: One laboratory method to detect for COVID-19 infection is via real time RT-PCR. The process starts…
A: Real-time RT–PCR is a nuclear-derived test for detecting the presence of RNA or DNA of the pathogen…
Q: 3 types of common coronavirus tests (RT-PCR for viral RNA with Taqman detection, lateral flow…
A: Coronavirus has spread like a wildfire and the disease caused by it has turned into a global…
Q: Can you develop a siRNA-based technology to treat COVID-19? If so, please provide the details on the…
A: YES,according to me this insight seams to be reasonable. siRNA or small interfering RNA is a non…
Q: I understand that microarrays are being used to define the molecular abnormality and the prognosis…
A: Leukemia and types
Q: escribe which human cell machinery the SARS-CoV-2 uses in order to rep
A: Some useful terms : SARS ( Severe acute respiratory syndrome), CoV ( covid 19) ACE 2 (…
Q: late the initial titre of the sample viral stock if the followir Show clear sample calculations. bda…
A: Introduction Infections that contaminate prokaryotic creatures, called bacteriophages or basically…
Q: The complete coronavirus genome sequence from the first known patient infected with SARS-CoV-2 was…
A: Coronaviruses have a global distribution and are known to infect a variety of human and animal…
Q: Discuss how recombinant DNA technology could be used to develop a diagnostic method for the novel…
A: Introduction The combining of DNA molecules from two distinct species is known as recombinant DNA…
Q: Transmission studies suggest that there are at least three (3) different variants of the SARS-CoV-2…
A: SARS-CoV2 is a virus consisting of four main structural proteins called spike, membrane, envelop,…
Q: You discover a new bacteria and are interested in mapping its genome with respect to 10 genes. You…
A: The conjugation experiment data is given in the question. The genes are transferred to the recipient…
Q: Explain its principle; its advantages and disadvantages.
A: PCR relies on DNA polymerase's ability to manufacture new DNA strands that are complementary to the…
Q: What kind of DNA is actually used to make the spots for analysis for the Vicrochip DNA microassay?
A: DNA (deoxyribonucleic acid) microarray or DNA chip is a technique that is used to analyze the…
Q: Two of the vaccines being used to prevent SARS-CoV-2 infection (the virus causing COVID-19 disease)…
A: Introduction :- A vaccine is a biological preparation that provides active acquired immunity to a…
Q: he Pfizer Covid-19 vaccine is one of the vaccines currently being rolled out for mass vaccination in…
A: Vaccine -- First human vaccine were used against virus was attenuated viruses .Vaccine can be…
Q: Find out if BamHI is a good restriction endonuclease for cutting the SARS-COV-2 genome? Give…
A: Endonucleases are enzymes that cut within the sequence of the DNA. Restriction endonucleases…
Q: 3.) A number of publications explain an expanding knowledge on the genetics and genomics of…
A: SARS-CoV-2: SARS-CoV-2 is the virus responsible for causing the COVID-19 pandemic that began in…
Q: pZERO®-1 is a 2808 bp cloning vector from Invitrogen. This vector allows effective selection of…
A: The PET sequences are simentensously traced to the genome assembly to define the boundaries of…
Q: You have constructed a cDNA library in a phagemid vector. Describe how you would screen the library…
A: Phagemid vectors are plasmid DNA [Deoxyribonucleic acid] containing a small portion of the genome of…
Q: How could sequencing many SARS-CoV-2 genomes be used to track how the virus has changed over time…
A: Mutation It refers to the alteration of the sequence of the DNA or RNA in organisms. These mutations…
Q: Research suggests that there are several SAR-CoV-2 variants circulating in the Jamaican population.…
A: SARS-CoV-2 The SARS-CoV-2 virus is responsible for causing Coronavirus Illness (COVID-19), an…
Q: Discuss recombinant techniques that could be used for the detection of the new coronavirus SARS-CoV2…
A: INTRODUCTION: We all now know a little bit about SARS-CoV2 virus after the pandemic of COVID -19.…
Q: You are a graduate student working to construct a single gene knockout library of Leptospiria…
A: * That constructed a single gene knockout library of Leptospiria kirschneri one the causative agents…
Q: Bacteriophage P22 was used in generalized transduction experiments to infect the Salmonella…
A: Transduction can be defined as a genetic engineering method that involves the insertion of foreign…
explain the full method you would use to sequence the full genome of SARS-Cov-2
Step by step
Solved in 2 steps
- How real time RT-PCR can be used in detecting SARS-nCov2 virus? Please explain at your own words.The SARS-CoV-2 genome is a polycistronic genome. What does the term polycistronic refer toConsidering counting rules, calculate the initial titre of the sample viral stock if the following plaques were counted in a plaque assay. Show clear sample calculations. Table 1: Plaque assay counts for lambda phage stock titre enumeration of average concentration (PFU/mL) Total dilution of viral stock 1/13500000 1/135000000 1/135000000 plated Volume diluted viral stock 0.1 0.1 0.1 plated (mL) PFU replicate 1 TNTC 275 25 Calculated concentration (PFU/mL) replicate 1 PFU replicate 2 TNTC 239 23 Calculated concentration (PFU/mL) replicate 2 Average concentration (PFU/mL)
- The complete coronavirus genome sequence from the first known patient infected with SARS-CoV-2 was published on January 10th 2020, just a short time after the patient had been hospitalized in China with respiratory symptoms. They did this so quickly using nanopore sequencing. Scientists compared the genome sequence from the new coronavirus (SARS-CoV-2) with the genome sequences from several other coronaviruses and they did identify a sequence unique to the SARS-CoV-2 strain. This unique sequence was in the N gene of the viral genome. What protein is made from the N gene of the virus?3.) A number of publications explain an expanding knowledge on the genetics and genomics of SARS-CoV-2 and its implications for understanding COVID-19. Why should a healthcare professional need to have a solid understanding of these basic genetics and genetic engineering terms? How would this knowledge help you in your nursing practice? 4.) Scientists and researchers are using genetic engineering rather than traditional methods in developing COVID-19 vaccines. In a tabular format give at least 2 examples of vaccines that uses genetic technology and describe each in terms of: a. Target Population b. Vaccine Efficacy C. Vaccine administration d. Possible side effects Storage requirements e.In the extraordinary times in which we live, biotechnology is playing a vital role in helping to identify and contain coronavirus, SARS-CoV-2, outbreaks around the world. There are three tools that are fundamental to many areas of biological research: DNA isolation, polymerase chain reaction (PCR) and gel electrophoresis. Write an essay that outlines the key steps in testing for active SARS-CoV-2 (the coronavirus responsible for causing Covid-19) in a patient. Begin with a brief description of how the sample is collected and follow with a description of each of the biotechnology procedures that are used to determine if the patient’s sample contains SARS-CoV-2.
- Discuss recombinant techniques that could be used for the detection of the new coronavirus SARS-CoV2 (ss RNA genome) which causes COVID-19 disease.Refer to the figure. What method would you use if you wanted to determine the sequence of the cDNA of the peak protein? Briefly explain the sequencing methodDifferentiate Invitrogen pCR® II-TOPO® Vector from pBR322 Vector.
- You are counting plaques on your plaque assay plates made from serial dilutions of your high titer lysate. Your 10-5 plate has 615 plaques although some are butting up against each other so it is difficult to get an accurate count. Your 10-6 plate has 42 plaques, and your 10-7 plate has only 1 plaque. Which plate would probably yield the most accurate titer calculation of your phage and why is it more trustworthy than the others?How is polymerase chain reaction used as a diagnostic test for COVID-19? PCR serves as a molecular test to detect the SARS-CoV-2 virus's genetic material. PCR serves as an antigen test that detect specific proteins from the virus PCR is used to kill the SARS-CoV-2 virus in a patient's sample. PCR is used to quantify specific proteins in the SARS-CoV-2 virus.What ingredients are used to develop a Suppositories dosage form? Justify the uses of each ingredient. Please answer at your own words.