Explain how viruses can be isolated using filtration State that viruses must be cultured in cells. Common choices: bacteria, mammalian cells, chicken eggs Explain how a plaque assay works Given PFU data, be able to calculate density of virus in a sample in PFU/mL Define clinical trial and state where you can find info about clinical trials Explain how viruses are used in gene therapy

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Q: Vivid outline and Standard operating procedure of Viral hemagglutination inhibition test.

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Q: how a RT-PCR test could be set up for the detection of this virus

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Q: Describe the limitations of RF Agglutination test

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Q: Explain the main defects of using animal models for viral growth and cultivation.

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Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.