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- Which of the following methods can be applied to detect the infectious virus Immuno-electron microscopy Plaque assay Western blot Hemagglutination inhibition Quantitative PCRVivid outline and Standard operating procedure of Virus neutralization test.Write a lab report on enumeration of lytic viruses : the plaque assay including the sections: introduction, Materials and Methods, Results and Discussion, and citing peer reviewed sources use this data for results section Group Number Number of Plaques Dilution Factor of Plate Original Concentration of Phage 1 58 10^3 5.80E+04 2 37 10^3 3.70E+04 3 44 10^3 4.40E+04 4 63 10^3 6.30E+04 5 36 10^2 3.60E+03 6 65 10^3 6.50E+04 7 260 10^2 2.60E+04 8 39 10^3 3.90E+04 9 178 10^2 1.78E+04 10 192 10^2 1.92E+04 11 274 10^2 2.74E+04 12 224 10^2 2.24E+04 Class Average 3.52E+04
- Give advantages and disadvantages of using commercially available kits for plasmid extraction over conventional alkaline lysis technique.What other test can be used to confirm the results obtained for a TEV infected tomato in an ELISA test? Name and very briefly describe one other test. Give the reason for your choice.One laboratory method to detect for COVID-19 infection is via real time RT-PCR. The process starts with nasopharyngeal or oropharyngeal swabbing to get a sample. Explain concisely how this technique generally works and how it can be used for testing.
- Disrobe laboratory method Western Blot used to evaluate the immune response, describe how it works in detail, Also, please provide at least one pathogen your method of analysis is used to detect.Many ELISAs that are used to identify the presence of antibody to viruses have a 3rd control (along with negative and positive). In between the rows of wells coated with antigen, are rows of cells coated with tissue culture used to grow the virus. In other words, patient serum is added to wells that contain antigen along with wells that contain tissue culture. Why do you think this might be necessary?Considering microbial identification methods, to which category would the following method belong based on what it measures? * Examination of the shape and height of bacterial colonies growing on a plate Genotypic O Biochemical O Phenotypic Serological
- What are the basic differences between the three plating methods? Fill up the following table. Streak Pour Spread Equipment/ Materials used in immobilizing and separating cells Purpose(s) (isolation, enumeration, both) Type of colonies (surface, subsurface, both) Advantage DisadvantageDuring the incubation step of the plaque assay, viral replication leads to host cell lysis. This allows the freshly-released virions to diffuse through the media and infect new hosts. The amount of agar added to the top agar is 7 g per liter rather than the 16 g of agar per liter for most solid media, including the bottom agar used in this assay. How would it change the plaque assay results if bottom agar was used in place of the top agar in this assay? a- The diameter of the plaques would be smaller. b- The diameter of the plaques would not change. c- The diameter of the plaques would be larger. (2) A researcher measured the viral titer of PhiX174 in fish tank water, untreated sewage water, and unpasteurized apple juice. Which sample do you predict will have the highest titer on this page? a- Fish tank water b- Unpasteurized apple juice c- Untreated sewage waterRefer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000