DNA microarrays (gene chips) are only capable of monitoring the expression of one gene at a time. O True O False

Q: What would three possible reasons be that a PCR wouldn't work after cloning a TAQ sequence into a…

A: Even though you've verified Taq protein expression through SDS-PAGE, your PCR might still fail for a…

Q: In 1997, Dolly the sheep was cloned using somatic cell by Kary Mullis. Lütfen birini seçin: O a.…

A: false

Q: In recombinant DNA technology, restriction enzymes cleave the DNA at a specific site known as:

A: Restriction enzyme, also known as restriction endonuclease, is a bacterial protein that cleaves DNA…

Q: Why is this technique used?

A: All cells must synthesize some proteins for their cellular function. The instruction for protein…

Q: Which temperature is Taq polymerase's optimal temperature O 20 degrees C 72 degrees C 55 degrees C O…

A:

Q: True or False: 1. The 6X HIS tag is required for a eukaryotic protein to be expressed in E. coli 2.…

A: Polyhistidine tag is added to a protein and helps in its purification. A recombinant protein can…

Q: Which of these is not a tool for comparing DNA sequences?   BLAST Fasta PLINK A dotplot e.g.…

A: The amount of data generated by next-generation sequencing platforms is enormous. The information…

Q: When molecular biologists carry out a PCR, they have a range of different DNA polymerase enzymes on…

A: Polymerase chain reaction (PCR) is a frequently used approach for rapidly producing millions or even…

Q: Which of these is not a tool for comparing DNA sequences? PLINK Fasta BLAST A dotplot e.g. dotlet

A: DNA polymerase is an enzyme. A primer is a single-stranded DNA fragment that binds to template DNA…

Q: You discover a new gene cluster from a strain of the bacterium Streptomyces. The domains of the…

A: Polyketides(PKs) and non-ribosomal peptide hybrids(NRPS) are important class of natural products,…

Q: PCR is used for cloning DNA and after about 30 cycles, the number of copies DNA will have increased…

A: The enzyme DNA polymerase which is used for PCR reactions is called Taq DNA polymerase which is…

Q: Which of the following are common types of data/analysis that are used in bioinformatics. O DNA…

A: Disclaimer: As per Bartleby guidelines, we will answer only the first question. P, lease repost the…

Q: Even though RNA degrades quickly (unstable) and is tedious to isolate compared to DNA isolation, why…

A: As structure of RNA is similar to DNA only difference that RNA is single stranded and DNA is double…

Q: To quantify the transcription product of a specific gene in E. coli, you may use (select all correct…

A: Introduction The Process Of Transcribing A Piece Of DNA Into RNA Is Known As Transcription.…

Q: The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also…

A: Chromosomes are carrier of deoxyribonucleic acid (DNA). DNA is the genetic material. Simple sequence…

Q: If the primer that you design anneals to two different regions of the DNA to be sequenced, the…

A: A primer is a short sequence of nucleic acids that serves as a starting point for DNA synthesis.…

Q: Which of the following will MOST LIKELY yield a construct containing an insert in the correct…

A: A vector construct, also known as a cloning vector or expression vector, is a DNA molecule that is…

Q: Correctly match the material/supply to its role (job) in genetic engineering. vcuts gene and…

A: Answer 2. The correct match and its role (job) in genetic engineering is 1. Cuts gene and recipient…

Q: What does BLAST stand for? Basic Local Alignment Search Tool Basic Local Alignment SequenceToo Best…

A: The best option is (a) Basic Local Alignment Search Tool.

Q: Real-time PCR is used in many transcription? Select one or more: O a. Gene transcription qu b. Ob.…

A: Note- According to the guidelines only one question can be answered at a time. Hence, the first…

Q: lo study the function of any gene of interest you would perform the loss and gain of function…

A: "Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: You work in an environmental microbiology research lab, and your project is to design a recombinant…

A: Our task as research assistants in an environmental microbiology lab is to create a recombinant…

Q: however it is still unclear that the function of th gene is and the lab director asks you for…

A: Previously getting the idea of the genome of any organism was very difficult but, Recombinant DNA…

Q: Select all that are true about DNA gel electrophoresis O This technique can be used to separate DNA…

A: The gel electrophoresis is routinely used technique in molecular biology laboratories that helps in…

Q: Design forward and reverse primers to amplify this ENTIRE locus by PCR. Design each primer to be 10…

A: The forward and reverse primers are both important components used in PCR for amplifying a target…

Q: ori Clo fcoR v OFP Below is the plasmid map of pGLO which contains the Green Fluorescent Protein…

A: pGLO:   pGLO is an engineered plasmid that contains genes for beta- Lactamase.  Beta-lactamase…

Q: Once you have idenified a gene that you suspect is responsible for the disease, you clone the gene…

A: Out of all the options given -  Option - Northern Blot , is incorrect because northern blotting is a…

Q: How would we amplify the target DNA associated with PTC tasting? O We use restriction enzymes to…

A: PCR polymerase chain reaction, is the most useful invitro techniques nowadays to amplify double…

Q: [GAPDH] is varied per sample. Cannot be determined [GAPDH] is comparable per sample. [GAPDH] is not…

A: PCR :It stands for polymerase chain reaction.It is a rapid and versatile in vitro method for…

Q: i have a gene (3222bp) i want to put it into a expression vector (pet 28b+),when i did pcr i flanked…

A: Introduction  Gene cloning is the technique to produce the exact copies of the desired gene by…

Q: Match each of the terms in the left column to the bestfitting phrase from the right column.a. exome…

A: a. Exome: It is the part of a genome that contains all the exons.b. de novo gene: These are the new…

Q: One improvement of CRISPR-Cas9 technology over the classical recombinant DNA technology is its…

A: CRISPR/Cas9 is very fascinating technology discovered till date. Its a gene editing tool widely used…

Q: RNA microarrays can be used to determine which genes are turned on Select one: a. False b. True

A: The gene expression is the production of RNA from a particular DNA (gene) by the process of…

Q: In this diagram of a DNA electrophoresis gel, which sample contains the smallest molecules? АВСD в с…

A:

Q: ..... doesn't use for gene expression at global level     Microarrays maldi tof mass…

A: Ans : maldi tof mass spectrometry

Q: Which of the following steps is NOT involved in gene cloning? Lütfen birini seçin: O a. Transferring…

A: Gene cloning is the technique of isolating and forming copies of gene of interest.

Q: RNA sequencing can detect DNA mutations typically easier to analyze can detect transcripts with very…

A: Microarray technology is used to measure the expression levels of thousands of genes simultaneously,…

Q: explain the methods of how we could clone wooly mammoths

A: Cloning is the process of producing genetically identical individuals either naturally or…

Q: What is a contig?

A: Chromosomes are long thread-like structures that carry coded genetic information in the form of DNA.…

Q: Synthesis of hybrid genes can occu Directed mutagenesis O Using overlap primers O RT-PCR

A: PCR technique of amplification of a given molecule of DNA using taq polymerase enzyme.

Q: During the process of genetic transduction in bacteria, two living cells exchange segments of their…

A: Transduction Unlike transformation in which the naked DNA is transferred in Transduction DNA is…

Q: Which of the following tools should be used in identifying a mutation in a sequence compared to a…

A: Gene mutations, and alterations in the DNA sequence, play a fundamental role in shaping life and…

Q: The Centers for Disease Control and Prevention (CDC) program PulseNet uses genomic fragments that…

A: Pulsenet uses highly multiplexed amplicon sequencing (HMAs) panels for surveillance subtyping. It…

Q: What is DNA fingerprinting? what is its application/s (legally and medically) and limitation/s?

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Which of these is not a tool for comparing DNA sequences? O PLINK O A dotplot e.g. dotlet O Fasta O…

A: There are different tools in bioinformatics that help in sequencing and comparing the DNA sequences.…

Q: What sorts of laws (if any) do you think should be passed regarding the confidentiality of genetic…

A: Introduction Genetics is the study of the transfer of certain traits (eye color, hair color) and…

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.