Discuss the effect of EDTA on ca absorbance in AAS experiment
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Discuss the effect of EDTA on ca absorbance in AAS experiment
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- What might happen if you omit potassium tellurite in using GCM?Determine the amount of glucose in the unknown sample by plotting a standard curve of Absorbance at 620 nm on Y-axis and μg of glucose on the x-axis.The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.
- Guanosine (C10H13N5O5) in solution has a maximum absorbance at a wavelength of 275 nm. The molar extinction co-efficient at this wavelength is 84M−1cm−1and the path length is 24.7 cm. Through the use of a spectrophotometer, it is found that the that A275= 1.48. What is the concentration of the guanosine solution in grams/litre? Molecular weights (g/mol): C-12, H-1, N-14, O-16 Select one: A. 0.201869 g/L B. 0.435188 g/L C. 0.059919 g/L D. 0.294046 g/L E. 0.000713 g/LThe concentration ofacetylcholine (a neurotransmitter) in a sample can be determined from the pH changes thataccompany its hydrolysis. When the sample is incubated with the enzymeacetylcholinesterase, acetylcholine is converted to choline and acetic acid, whichdissociates to yield acetate and a hydrogen ion: In a typical analysis, 15 mL of an aqueous solution containing an unknown amount ofacetylcholine had a pH of 7.65. When incubated with acetylcholinesterase, the pH of thesolution decreased to 6.87. Assuming there was no buffer in the assay mixture, determinethe number of moles of acetylcholine in the 15 mL sample.how to calculate these? can you give me the steps or the equations for that? 2ul St.1 St.2 St.3 St.4 Absorbance 595nm 0.926 0.444 0.421 0.462 0.932 0.412 0.407 0.393 Ç Average Abs Corrected Abs 595nm 595nm 0.929 0.428 0.414 0.4275 0.5615 0.0605 0.0465 0.06 [Protein] (µg/ml) 103.98 11.20 8.61 11.11 Volume (ml) 20 40 8 4 [Total Protein] (ug) 2079.63 448.15 68.89 44.44
- Why are quantitative absorbance measurements made at lambda max?Example The standard half electrode potential for Fe²+ vs SHE is 0.77 V. What is the value of Ee at T = 298K when: [Fe³+] = 0.2 mol l-1 [Fe2+] = 0.05 mol 1-1The protein calcineurin binds to the protein calmodulin with an association rate of 8.9 × 103 M−1 s −1 and an overall dissociation constant, Kd, of 10 nM. Calculate the dissociation rate, kd , including appropriate units.
- A Br selective electrode is applied for measuring bromide ions in saline solution. Cl' ions were found to interfere, with a kPot = 0.0050. %3D After calibration, the ISE equation was determined to be y = -70 mV - 59.2mV*log(x). If we measure a potential of 93 mV, and the [CI]=0.100 M, what was the [Br'] in units of M? O 0.00821 O 0.100 O 0.0050 O 0.123 O Correct answer not found in other optionsCalculate the effective quantity (g) of sodium chloride related to tonicity in 100ml of an intravenous fluid labeled "5% dextrose in 0.45% sodium chloride," and indicate whether the solution is isotonic, hypotonic or hypertonic. The answer is Hypertonic and 1.35g NaCl but I don't understand how to get the answer.The electronic excitation method in fluorescence and absorption spectroscopy is the same. What are some of the variations between using a fluorimeter to perform an excitation scan and an absorption scan? Do you think the two spectra should be similar? Describe. More info attached like protocol if needed