Direction: Encircle the letter of the correct answer. 1. It is a double-stranded , self replicating, circular DNA molecule present in bacteria which is widely used as a gene cloning vector. A. Cosmid B. Plasmid. C. Phagemid. D. Genome In plant genetic engineering, which of the following acts as vector? Agrobacterium lumefaciens. Receipt plant cell. 2. А. B. Gene of interest D. Ti-plasmid Which of the following illustrates breeding? A farmer choose a breed of cow for greater milk production The use of bacteria in order to produce human insulin. С. 3. A. В. The insertion of cloned genes to plant cells. All of the above. D. Being pest resistance is one of the traits that are being introduced to plants like corn and eggplant with the insertion of Bt-toxin gene to plant cell. What method is use when a gene is inserted to plants? A. Biolistic C. Plasmid insertion by heat shock treatment 4. B. Electroporation D. All of these
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
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