Design the simple process scheme diagram for (fermentation and recovery of glutamic acid from palm waste hydrolysate by Ion-exchange resin column) Note :The diagram should involve arrow diagram
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Design the simple process scheme diagram for (fermentation and recovery of glutamic acid from palm waste hydrolysate by Ion-exchange resin column)
Note :The diagram should involve arrow diagram.
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- Based on your residing of this chapter, what would you expect to be the most immediate effect oil glycolysis, if the steady-state concentration of gJucose-6-P were 8.3 mM instead of 0.083 mM?Anaerobic production of ethanol by Saccharomyces cerevisiae is associated with yeast growth. The steady-state growth kinetics of yeast follows Monod. It is intended to produce 10 kg/h of ethanol in an ideal CSRT-type fermenter, fed by a solution of 50 g/L of glucose. Size the fermenter for this purpose (calculate its working volume), setting the biomass concentration at the exit of the fermenter at 6.9 g/L.Anaerobic production of ethanol by Saccharomyces cerevisiae is associated with yeast growth. The steady-state growth kinetics of yeast follows Monod. It is intended to produce 10 kg/h of ethanol in an ideal CSRT-type fermenter, fed by a solution of 50 g/L of glucose. Size the fermenter for this purpose (calculate its working volume), setting the biomass concentration at the exit of the fermenter at 6.9 g/L.Note: CSTR = Continuous Stirred Tank ReactorData: μmax = 0.1 h-1; Ks = 0.5 g/L; YX/S = 0.15; YP/S = 0.45.(answer: V = 5435 L)
- Make single cell protein(SCP) animal feed from cassava effluent (mainly starch). The effluent is obtained from pressing of crushed fresh cassava pulp generated by gari processing factory. Using Saccharomyces cerevisiae strain; (a) Select and describe fermentation process for the SCP production stating the conditions involved (b) State the monitory measures you would consider for the yield of production (c) State how the product can be verified for animal feedingproduce single cell protein(SCP) animal feed from cassava effluent (mainly starch). The effluent is obtained from pressing of crushed fresh cassava pulp generated by gari processing factory. Using Saccharomyces cerevisiae strain; (a) Select and describe fermentation process for the SCP production stating the conditions involved (b) State the monitory measures you would consider for the yield of production (c) State how the product can be verified for animal feedingcreate a detailed flow chart or diagram that will illustrate all the important features of the glycolytic pathway. I want you to place in your flow chart or diagram the following important information like: 1. the reactants and products of each step, 2. enzymes involved in each step, 3. important by-products generated in some steps, 4. type of the reaction of each step, 5. and the outline of the two stages of the pathway. Also, discuss briefly (4 sentences) how Galactose and Fructose enter the glycolytic pathway.
- i) Re-arrange the Michaelis Menten equation so it involves the ratio [S]. Show all steps beginning Km noting any assumptions or required conditions. Km ii) Calculate the ratio [lo for the case when the rate of product formation is 68% of Vmax and the substrate is in great excess. d[P] dt : k₂ with = [E],[S] Km+[S]' [S]o Km iii) Explain, in a few sentences, why the ratio determines the ratio V Vmax V Vmax Begin by explaining the meaning of stating simply "it's the ratio...." is not sufficient. Include in your explanation the factors that effect v and Vmax. Consider what factors make v different from or equal to Vmax. Consider what Km represents concerning processes involving ES. " iv) Calculate KM at 310K at given the following rate constant information: k₁ = 17 s-¹M-1 at 300K with A = 7300 s-¹M-1 K-1₁ 6 s¹ at 300K with A = 14500 s -1 k₂ = 31 s¹ at 300K with A = 600 s-¹Match each of the enzymes involved in de novo pyrimidine synthesis with the correct description: _______ Dihydroorotate dehydrogenase _______ UMP synthase_______ Carbamoyl phosphate synthetase II _______ TMP synthase _______ CTP synthetase_______ Aspartate transcarbamoylase _______ PRPP synthetase (A) attaches an aspartate to an activated carbamoyl molecule(B) bifunctional enzyme that combines a nitrogenase pyrimidine ring to an activated ribose(C) catalyzes the formation of the activated ribose sugar(D) uses glutamine to add an amine to a preformed nucleotide(E) produces the first cyclic nitrogenase ring during de novo pyrimidine synthesis(F) combines bicarbonate and nitrogen from glutamine in the first step of pyrimidine ring synthesis (G) uses a folate derivative to add a methyl group to a preformed nucleotideOne of the steps in psilocybin biosynthesis is the phosphorylation of 4-hydroxytryptamine By the enzyme Psik as shown below, that involves the hydrolysis of ATP. NH₂ OH HO Psik HO of A& ADP 4-hydroxytryptamine ATP norbaeocystin The standard free energy changes for each reaction is shown in the Table below. J reaction 4-hydroxytryptamine + P₁ → norbaeocystin ATP → ADP+ Pi (b) What is the equilibrium constant at 37 °C? AG 27.7 kJ/mol -32.2 kJ/mol (a) Write the net reaction. What is the standard free energy change for the net reaction? (c) In the cell, the concentration of ATP is 3.1 mM, the concentration of P; Is 5.90 mM, and the Concentration of ADP is 220 μM. What will AG Be if the concentration of norbaeocystin Is always kept at 1/100 of the concentration of 4-hydroxytryptamine?
- Manufacture single cell protein(SCP) animal feed from cassava effluent (mainly starch). The effluent is obtained from pressing of crushed fresh cassava pulp generated by gari processing factory. Using Saccharomyces cerevisiae strain; (a) Select and describe fermentation process for the SCP production stating the conditions involved (b) State the monitory measures you would consider for the yield of production (c) State how the product can be verified for animal feedingGraph these two datas with correct y axis ( label absorbance and time) of the amylase effects on carb cutter and compare the two graphsThe yeast S.cerevisae is cultivated for ethanol production in a 10000 L fermenter operated continuously at steady state. The reactor is fed by a 50 g/L of sucrose, with a feed flow of 400 L/h. Assuming that the yeast growth kinetics follow the Monod model and using the following information:Data: μmax= 0.1h-1 ; Ks=0.5g/L; Yx/s=0.15 g/g; Yp/s=0.45g/g. a) What are the concentrations of biomass, sucrose and ethanol at the exit of the fermenter?b) Calculate the productivity in ethanol (Pp) and sketch the curve of variation of Pp vs. dilution rate.c) In terms of ethanol productivity, would it be more or less advantageous if the reactor were operated with a feed rate of 1200L/h? Justify.