Demonstration of transposition through an RNA intermediate FIGURE 15-14 A Ty element is altered by adding an intron and a promoter that can be activated by the addition of galactose. The intron sequences are spliced before reverse transcription. Plasmid with Ty Coding region LTR LTR Ty element Galactose-inducible promoter Intron from another gene Add galactose. Primary transcript Splicing MRNA Reverse transcription Insertion New copy of Ty element lacking introns
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The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
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If the transposable element in Figure 15-14 were a DNA
transposon that had an intron in its transposase gene,
would the intron be removed during transposition?
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