Estimate (from your data) the [Protein] of your unknown based on comparing to the standards. Include the correct unit. This estimate should be an integer (no decimals). Cuvette (ug/ml BSA)BLANK5E101520* Repeat readings if needed.*A595Should be zeroCuvetteUnknownRepeat readings if needed.055·078•117• 1359. Show your data to your professor. When you have a good standard curve, your professor willgive you a sample of unknown protein concentration. Treat this sample in the same way as yourBSA unknowns and determine the Absorbance at 595nm.A595A595 *Should be zero0.103055•078117135.A595aft0.103.

Answered: Image /qna-images/answer/7e07fb25-b5f7-4383-b75c-d2ed1f53e342.jpg

Cuvette (ug/ml BSA) BLANK 5 10 15 20 * Repeat readings if needed. A595 Should be zero Cuvette Unknown neat readings if needed. 055 ·078 •117 135 A595 * 9. Show your data to your professor. When you have a good standard curve, your professor will give you a sample of unknown protein concentration. Treat this sample in the same way as your BSA unknowns and determine the Absorbance at 595nm. 0.103 A595 Should be zero 055 • 078 •117 • 135 A595 * 0.103.

Cuvette (ug/ml BSA) BLANK 5 10 15 20 * Repeat readings if needed. A595 Should be zero Cuvette Unknown neat readings if needed. 055 ·078 •117 135 A595 * 9. Show your data to your professor. When you have a good standard curve, your professor will give you a sample of unknown protein concentration. Treat this sample in the same way as your BSA unknowns and determine the Absorbance at 595nm. 0.103 A595 Should be zero 055 • 078 •117 • 135 A595 * 0.103.

Chemistry: The Molecular Science

5th Edition

ISBN:9781285199047

Author:John W. Moore, Conrad L. Stanitski

Publisher:John W. Moore, Conrad L. Stanitski

Chapter13: The Chemistry Of Solutes And Solutions

Section: Chapter Questions

Problem 57QRT

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