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Cuvette (ug/ml BSA) BLANK 5 10 15 20 * Repeat readings if needed. A595 Should be zero Cuvette Unknown neat readings if needed. 055 ·078 •117 135 A595 * 9. Show your data to your professor. When you have a good standard curve, your professor will give you a sample of unknown protein concentration. Treat this sample in the same way as your BSA unknowns and determine the Absorbance at 595nm. 0.103 A595 Should be zero 055 • 078 •117 • 135 A595 * 0.103.

Cuvette (ug/ml BSA) BLANK 5 10 15 20 * Repeat readings if needed. A595 Should be zero Cuvette Unknown neat readings if needed. 055 ·078 •117 135 A595 * 9. Show your data to your professor. When you have a good standard curve, your professor will give you a sample of unknown protein concentration. Treat this sample in the same way as your BSA unknowns and determine the Absorbance at 595nm. 0.103 A595 Should be zero 055 • 078 •117 • 135 A595 * 0.103.

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Estimate (from your data) the [Protein] of your unknown based on comparing to the standards. Include the correct unit. This estimate should be an integer (no decimals).
Cuvette (ug/ml BSA) BLANK 5 E 10 15 20 * Repeat readings if needed. * A595 Should be zero Cuvette Unknown Repeat readings if needed. 055 ·078 •117 • 135 9. Show your data to your professor. When you have a good standard curve, your professor will give you a sample of unknown protein concentration. Treat this sample in the same way as your BSA unknowns and determine the Absorbance at 595nm. A595 A595 * Should be zero 0.103 055 •078 117 135 . A595 aft 0.103.
Transcribed Image Text:Cuvette (ug/ml BSA) BLANK 5 E 10 15 20 * Repeat readings if needed. * A595 Should be zero Cuvette Unknown Repeat readings if needed. 055 ·078 •117 • 135 9. Show your data to your professor. When you have a good standard curve, your professor will give you a sample of unknown protein concentration. Treat this sample in the same way as your BSA unknowns and determine the Absorbance at 595nm. A595 A595 * Should be zero 0.103 055 •078 117 135 . A595 aft 0.103.
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