Below are responses generated using the isolated guinea-pig ileum preparation to determine a pA₂ value for the cholinergic antagonist homatropine. Responses are expressed in millimetres +/- SDM (n = 4). Plot the data and then determine by Schild analysis the pA₂ value and Schild slope. So that's TWO drawn graphs to plot on LIN/LOG paper and submit..not EXCEL graphs please. What conclusions can you draw; does the experiment/data fulfil the criteria for a valid Schild analysis? Acetylcholine ACH Alone Concentration ACH + ACH + ACH + Homatropine Homatropine Homatropine 3 x 10-⁹M 1 x 10-M 3 x 10-M [Molar] 3 x 109 3 +/- 1 6 x 109 8 +/- 3 No response 3 x 10-8 28 +/- 6 10 +/- 4 No response 6 x 108 38 +/-8 21 +/-9 5+/-2 No response 3 x 10-7 55 +/- 5 43 +/- 7 27 +/-9 10 +/- 4 6 x 10-7 56 +/- 5 52 +/- 3 38 +/- 7 20 +/-7 3 x 10-6 55 +/-4 53 +/- 6 43 +/- 6 6 x 106 56 +/-2 54 +/- 5

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Chapter1: Chemical Foundations
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Below are responses generated using the isolated guinea-pig
ileum preparation to determine a pA₂ value for the cholinergic
antagonist homatropine.
Responses are expressed in millimetres +/- SDM (n = 4).
Plot the data and then determine by Schild analysis the pA₂ value
and Schild slope.
So that's TWO drawn graphs to plot on LIN/LOG paper and
submit..not EXCEL graphs please.
What conclusions can you draw; does the experiment/data fulfil the
criteria for a valid Schild analysis?
Acetylcholine
ACH
Alone
Concentration
ACH +
ACH +
ACH +
Homatropine Homatropine Homatropine
3 x 10-⁹M
1 x 10-M
3 x 10-M
[Molar]
3 x 109
3 +/- 1
6 x 109
8 +/- 3
No response
3 x 10-8
28 +/- 6
10 +/- 4
No response
6 x 108
38 +/-8
21 +/-9
5+/-2
No response
3 x 10-7
55 +/- 5
43 +/- 7
27 +/-9
10 +/- 4
6 x 10-7
56 +/- 5
52 +/- 3
38 +/- 7
20 +/-7
3 x 10-6
55 +/-4
53 +/- 6
43 +/- 6
6 x 106
56 +/-2
54 +/- 5
Transcribed Image Text:Below are responses generated using the isolated guinea-pig ileum preparation to determine a pA₂ value for the cholinergic antagonist homatropine. Responses are expressed in millimetres +/- SDM (n = 4). Plot the data and then determine by Schild analysis the pA₂ value and Schild slope. So that's TWO drawn graphs to plot on LIN/LOG paper and submit..not EXCEL graphs please. What conclusions can you draw; does the experiment/data fulfil the criteria for a valid Schild analysis? Acetylcholine ACH Alone Concentration ACH + ACH + ACH + Homatropine Homatropine Homatropine 3 x 10-⁹M 1 x 10-M 3 x 10-M [Molar] 3 x 109 3 +/- 1 6 x 109 8 +/- 3 No response 3 x 10-8 28 +/- 6 10 +/- 4 No response 6 x 108 38 +/-8 21 +/-9 5+/-2 No response 3 x 10-7 55 +/- 5 43 +/- 7 27 +/-9 10 +/- 4 6 x 10-7 56 +/- 5 52 +/- 3 38 +/- 7 20 +/-7 3 x 10-6 55 +/-4 53 +/- 6 43 +/- 6 6 x 106 56 +/-2 54 +/- 5
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