Often the analyst will compare known quantities of analyte to unknown quantities of the material to be analyzed. This may be done in one of three ways: -use calibration curves; use standard additions; or use internal standards. The method of standard additions would be used when

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Often the analyst will compare known quantities of analyte to unknown quantities of the material to be analyzed. This may be done in one of three ways: -use calibration curves; use standard additions; or use internal standards. The method of standard additions would be used when

A) the quantity of sample analyzed or the instrument response varies from run to run.
 
B) the standard solutions and the unknown solution all have similar characteristics are unaffected by the other material in the sample.
 
C) the sample composition is unknown or complex and affects the analytical signal.
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