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Creatinine Determination:
Mention some specific methods based on principles other than Jaffe Reaction
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- State the principle that underlies the following biochemical tests: a. Voges-Proskauer testResults and Discussion: Sakaguchi Reaction: Samples: arginine , glycine, tryptophan Reagents: a-naphthol , Sodium Hypobromite (1ml Bromine water added to 1ml 10% NaOH, freshly prepared) -Mix 1 ml of 10% NaOH with 0.5 ml of the sample and add 1 ml of alpha-naphthol reagent. Then add drop by drop (about 5 drops) of the sodium hypobromite solution. Note the color formed.Identify the most cost effective enzyme purification method and provide brief explanations for this method. ▪Repeated freezing and thawing ▪Sonication ▪Homogenization by high pressure (French press) ▪Homogenization by grinding (bead mill) ▪Permeabilization by detergents (e.g. Triton X-100) and/or enzymes (e.g. lysozyme)
- State the principle that underlies the following biochemical tests: a) Methyl red testSketch the appearance after visualization of a protein mixture containing the seven proteins (fibrinogen, y-globulin, collagen, ovalbumin, myoglobin, hemoglobin, insulin) when subjected to two-dimensional (2D) gel electrophoresis.Give one advantage and one disadvantage of pharmaceutical solid dosage forms. Explain each advantage and disadvantage thoroughly.
- State the principle that underlies the following biochemical tests: a) Methyl red test b) Voges-Proskauer testDetermine the sequence of the decapeptide given the following information below. The decapeptide was hydrolyzed and confirmed to contain these amino acids: E, F, K, M, Q, S, T x 3, W Edman degradation product of the decapeptide: T trypsin fragments: a tetrapeptide (F,K,Q,T) and a hexapeptide (E,M,S,2T,W) chymotrypsin fragments: free S, a dipeptide (F,T) and a heptapeptide (E,K,M,Q,T,T,W) CNBr treatment of the heptapeptide (E,K,M,Q,T,T,W) yielded a tetrapeptide (K,T,M,Q) and a tripeptide (E,T,W) Edman degradation product of (E,T,W): E Use one-letter abbreviations only. One amino acid per blank.Using DEAE-cellulose as ion exhange resin, indicate the starting and ending pH for the narrowest experimental pH range used to seperate an amino acid mixture consisting of Ala, Cys and His Starting pH: Ending pH:
- Trade Names of the Pexidartinib (international and national) and write Generic name/ Chemical name:Answer the following questions: 3) Provide some possible errors% in the experiment of synthesis of cyclohexene 4) Explain unsaturation test with chemical reactions in the experiment of synthesis of cyclohexene |(Ctrl) -A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show working