Could the PCR sample loaded on lane 4 be considered a successful PCR? The image displays an agarose gel electrophoresis result, which is used to separate DNA fragments based on size. The gel is visualized under UV light, and the orange bands represent DNA fragments stained with a fluorescent dye.**Lanes:**- **Lane 1**: Contains a DNA ladder, used as a molecular weight marker for estimating the size of DNA fragments in other lanes.- **Lanes 2-7**: Display DNA samples that have been run on the gel. Each lane shows different band patterns, indicating the presence of DNA fragments of varying sizes.**Explanation of Bands:**- The bands visible in each lane correspond to DNA fragments. The position of each band is determined by its size; smaller fragments move further down the gel.- The brightness of the bands can indicate the concentration of the DNA. Brighter bands typically represent higher concentrations of DNA.- Lane 1 is typically used to compare the size of unknown DNA fragments in other lanes. The asterisk (*) denotes the negative electrode side of the gel, indicating the direction of electrophoresis is from top to bottom. This setup is standard for electrophoresis, as DNA has a negative charge and migrates towards the positive electrode at the bottom.

DNA cloning is a powerful technique but it is time-consuming and, in parts, difficult procedure. It…

Answered: Could the PCR sample loaded on lane 4…

Could the PCR sample loaded on lane 4 be considered a successful PCR?

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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