Consider the sequence below for amplification: GGCGTAGGCTGATCGTGGGCTCTAGGGGGCTGCTGCTGCTATTATGCTGGC TCTGGATTTTCCCCCGGCGGCGGCGCGCGCGCGCGCGTGTGTGTCCCACAC AGGGCCCCCAGGCTGGACCCACCCGGCCTGCTGCTCGAGCTGCGCTCCGGT GGTCGCGGTGCT Which of the following is least likely to assist you in optimizing the reaction? a. Increase the denaturation temperature b. Use a thermostable polymerase that has a lengthy half-life at 95°C c. Supplement the reaction mix with DMSO, formamide, urea, or Betaine d. Lower the primer annealing temperature e. Use a dNTP mix with 7-deaza-dGTP
Consider the sequence below for amplification: GGCGTAGGCTGATCGTGGGCTCTAGGGGGCTGCTGCTGCTATTATGCTGGC TCTGGATTTTCCCCCGGCGGCGGCGCGCGCGCGCGCGTGTGTGTCCCACAC AGGGCCCCCAGGCTGGACCCACCCGGCCTGCTGCTCGAGCTGCGCTCCGGT GGTCGCGGTGCT Which of the following is least likely to assist you in optimizing the reaction? a. Increase the denaturation temperature b. Use a thermostable polymerase that has a lengthy half-life at 95°C c. Supplement the reaction mix with DMSO, formamide, urea, or Betaine d. Lower the primer annealing temperature e. Use a dNTP mix with 7-deaza-dGTP
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:4.
Consider the sequence below for amplification:
GGCGTAGGCTGATCGTGGGCTCTAGGGGGCTGCTGCTGCTATTATGCTGGC
TCTGGATTTTCCCCCGGCGGCGGCGCGCGCGCGCGCGTGTGTGTCCCACAC
AGGGCCCCCAGGCTGGACCCACCCGGCCTGCTGCTCGAGCTGCGCTCCGGT
GGTCGCGGTGCT
Which of the following is least likely to assist you in optimizing the
reaction?
a. Increase the denaturation temperature
b. Use a thermostable polymerase that has a lengthy half-life at 95°C
c. Supplement the reaction mix with DMSO, formamide, urea, or
Betaine
d. Lower the primer annealing temperature
e. Use a dNTP mix with 7-deaza-dGTP
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