Consider the following data set: Sample Corrected Absorbance at 470 nm 100 ug/ml CuSO. standard 200 ug/mL CuSO. standard 300 ug/ml Cuso. standard 0.032 0.058 0.090 400 ug/ml CuSO. standard 500 ug/ml Cuso. standard CusO. Solution with Unknown Concentration 0.114 0.140 0.070 1. Plot (Using MS Excel) or draw by hand a standard calibration curve for samples 1 to 5. Determine the molar absorptivity using the absorbance of the 100 ug/mL CuSo, standard. (Note: Convert ug/ml to Molarity first using the molar mass of the standard which is 249.685 g/mol). l 2. Write the resulting linear regression equation and R of the plotted standard calibration curve. 3. Calculate the concentration of the "CuSO. Solution with Unknown Concentration" using the produced linear regression equation from the standard calibration curve and its absorbance at 470 nm.
Consider the following data set: Sample Corrected Absorbance at 470 nm 100 ug/ml CuSO. standard 200 ug/mL CuSO. standard 300 ug/ml Cuso. standard 0.032 0.058 0.090 400 ug/ml CuSO. standard 500 ug/ml Cuso. standard CusO. Solution with Unknown Concentration 0.114 0.140 0.070 1. Plot (Using MS Excel) or draw by hand a standard calibration curve for samples 1 to 5. Determine the molar absorptivity using the absorbance of the 100 ug/mL CuSo, standard. (Note: Convert ug/ml to Molarity first using the molar mass of the standard which is 249.685 g/mol). l 2. Write the resulting linear regression equation and R of the plotted standard calibration curve. 3. Calculate the concentration of the "CuSO. Solution with Unknown Concentration" using the produced linear regression equation from the standard calibration curve and its absorbance at 470 nm.
Chapter4: Least-squares And Calibration Methods
Section: Chapter Questions
Problem 9P
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