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Consider a mixture comprised of the proteins below: Protein that will most strongly bind to an anion exchange column is? Protein that will elute first in gel filtration chromatography is? Protein that will elute last in hydrophobic interaction chromatography is? Protein that will elute last in carbohydrate containing column is ?

Consider a mixture comprised of the proteins below: Protein that will most strongly bind to an anion exchange column is? Protein that will elute first in gel filtration chromatography is? Protein that will elute last in hydrophobic interaction chromatography is? Protein that will elute last in carbohydrate containing column is ?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Consider a mixture comprised of the proteins below:

Protein that will most strongly bind to an anion exchange column is?

Protein that will elute first in gel filtration chromatography is?

Protein that will elute last in hydrophobic interaction chromatography is?

Protein that will elute last in carbohydrate containing column is ?

protein A B C D MW (Kda) 55 80 300 760 Charge (ph 7.4) +1 -3 +2 -1 Affinity to a specific ligand NONE NONE NONE Carbohydrate % polarity 25 70 60 45
Transcribed Image Text:protein A B C D MW (Kda) 55 80 300 760 Charge (ph 7.4) +1 -3 +2 -1 Affinity to a specific ligand NONE NONE NONE Carbohydrate % polarity 25 70 60 45
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