Consider a mixture comprised of the proteins below: Protein that will most strongly bind to an anion exchange column is? Protein that will elute first in gel filtration chromatography is? Protein that will elute last in hydrophobic interaction chromatography is? Protein that will elute last in carbohydrate containing column is ?

Q: During SDS-PAGE,  the charge on glycine in the separating gel is ____________ while the charge on…

A: SDS-PAGE, or sodium dodecyl sulfate polyacrylamide gel electrophoresis, is a technique used to…

Q: Describe the method used to isolate vesicles for lipid analysis. How is C-Laurdan used to analyze…

A: Introduction: The below answer provides an explanation of two techniques used to study lipid…

Q: The figure shows binding curves for two proteins that bind the same ligand. Which binding curve…

A: Ligand binding curve: this is also called as saturation curve that shows the fraction Y of protein…

Q: Example of each sketched cell please. In the circles representing the microscope field of view,…

A: Tube 1: Distilled WaterAppearance: Swollen cellsWater Movement: Arrows pointing into the cellsTube…

Q: Isotonic, hypotonic, and hypertonic IV (intravenous) fluid solutions are widely used in the…

A: The solutions that are given to people are basically categorised on the amount of osmolarity that…

Q: how many ml of sterile water injection must be mixed with two 4 ml vials of sterile hypertonic NaCl…

A: Tonicity refers to the ability of a solvent to cause cells to shrink or swell. A semipermeable…

Q: The active drug substance given above contains a carboxylic acid group. Ionizable proton of…

A: The drug is considered an active chemical substance, which is generally a small biological molecule…

Q: What is the concentration of cells in the original cell suspension?

A: we can use the dilution formula:C1 V1 = C2 V2where:EXPLANATIONC1 is the initial concentration of the…

Q: How is it that smaller molecules move through a gel-filtration column more slowly than larger…

A: Various molecular and biological techniques are used today for the separation of materials in…

Q: During SDS-PAGE,  the charge on glycine in the stacking gel is ____________ while the charge on the…

A: SDS-PAGE is used to separate proteins based upon the relative mobility of charged species. Proteins…

Q: Time-release” drugs work by releasing a drug to the body at a constant rate such that the drug…

A: Time-release drugs use a special technology to release small amounts of the medication into a…

Q: Which of the following agents does NOT cause protein denaturation? Neutral pH Air Heat Alcohol None…

A: Introduction : Proteins are the nitrogenous organic compounds which are composed of one or more long…

Q: The graph below is a standard curve generated by plotting the distance travelled by the size…

A: The standard curve generated for an analysis involving separation of proteins along the SD-PAGE gel…

Q: Given the information:   Tube 6 contains 8.0 g/dL Tube 6 contains 0.5 mL of the dilute standard…

A: The measurement and computation of protein concentrations in a laboratory context are the subject at…

Q: In SDS page, the ________ proteins will move through the gel fastest and first, and in size…

A: SDS-PAGE is biochemistry technique used to separate proteins based upon the relative mobility of…

Q: What fluids are considered isotonic?    0.9% saline   5% dextrose   5% dextrose with 0.45%…

A: Isotonic solutions are those that have osmotic pressure equal to that of our body fluids such as…

Q: You have a stock solution of a tubulin antibody. You were instructed by your teaching assistant to…

A: In laboratory research and experiments, accurate dilutions of solutions are often required to…

Q: A microorganism was streaked onto 4 nutrient agar plates and the plates were incubated at different…

A: Nutrient agar  A type of general media that supports growth of wide variety of microorganisms.

Q: Where is there more solute in this image (inside the bag or outside the bag in the beaker)? 0.8M 0…

A: the molarity is defined as the number of moles of a solute dissolved in 1000 ml of the solution .…

Q: chemical(s) best at inhibiting both Gram- negative and Gram-positive bacteria [Choose ] [Choose ]…

A: Hi! Thanks for your question. As you have posted multiple questions and have not mentioned which one…

Q: (b) Why does solution pH affect separation of proteins via electrophoresis? (c) With reference to a…

A: Electrophoresis is a technique which enables the separation of proteins. Proteins in the sample get…

Q: Mannitol, a carbohydrate, is supplied as a 25% (w/v) solution. This hypertonic solution is given to…

A: Mannitol is a type of alcohol sugar formed from the monosaccharide carbohydrate, mannose. Mannitol…

Q: If you added 5 mL of a 20X tocopherol to 5 mL of cell culture at a density of 2.4 x 107 cells/mL,…

A: For calculating the concentration of a solution, the formula used is  M1V1=M2V2 Where, M1 =…

Q: HOW MANY of the following five items represent modes of protein denaturation? 1.) heating a protein…

A: Proteins are biomolecules composed of amino acids. Amino acids are joined together through peptide…

Q: Figure 3: ● • . ● KDa ● 97.4 66.2 45.0 ● 31.0 21.5 14.4 S-1 p-1 2.8 2-d S.3 p-3 This figure was…

A: The study of protein behavior under different conditions is a fascinating aspect of molecular…

Q: 10mL of 1x solution from a 2x stock solution.

A: Dilution formula - C1V1 = C2V2 C1 = concentration of stock C2 = final concentration V1 = volume of…

Q: Negatively charged proteins migrate towards the top of the gel backwards towards the positive…

A: The gel electrophoresis is a technique by which different biological samples including DNA, RNA and…

Q: You're trying to purify a protein with the aid of an ion exchange column. On the column, the protein…

A: The most commonly used technique to separate and purify proteins, nucleic acids, polypeptides and…

Q: What is the importance of acids and bases in our daily activities? State examples. What is the pH of…

A: Acids are the substances that donates H+ ions and have pH range between 1-6 Bases are those which…

Q: In the case of a protein suspension, which of the following statement/s is/are TRUE? I. The smaller…

A: Gel exclusion chromatography is a low resolution isolation technique that makes use of a neat…

Q: Dextrose 5% in water solution is an example of hypertonic solution.  True False?

A: Hypertonic solution is defined as the solution that contains high concentration of solutes than the…

Q: Standard curves You are setting up a standard curve for lysozyme. You have a stock vial containing 2…

A: Proteins are chains of amino acids bonded via amide bonds. Lysozyme is an antimicrobial enzyme that…

Q: Which among the following statements is correct? Group of answer choices Both statements are…

A: Chromatography is a technique used for separation of molecules based on the principle of…

Q: 1) If the pH of the rain drops from 6 to 5 due to air pollution, the rain has become: a) two time…

A: Acid rain caused when the rain is mixed with the volatile toxic compounds like sulfur dioxide and…

Q: Which of the following chromatographic techniques allows a faster elution of polar proteins? O…

A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…

Q: The energy required for diffusion of a solute across a cell membrane is: the kinetic energy of the…

A: As according to our guidelines, we are allowed to do a single question at a time, if you want second…

Q: You have a cellulose sack that is impermeable (i.e. NOT permeable) to starch molecules and is filled…

A: The ability of a solution to change the water content of cells and modify the cell volume is called…

Q: Five dialysis bags, impermeable to sucrose, were filled with various concentrations of sucrose and…

A: Five dialysis bags with varying sugar concentrations are provided. However, the bag's membrane is…

Q: Angiotensin Converting Enzyme (ACE) is the target of ACE inhibitors such as captopril that are used…

A: Peptides are composed of twenty standard amino acids. These twenty standard amino acids differ from…

Q: What is true about 'cracking' of emulsion? Select one: O a. The primary emulsion does not become…

A: Emulsion can be defined as the mixing of two or more liquids which are not immiscible (nonmixable or…

Q: For the following problem indicate the values for each part of the equation and solve for the…

A: The process of lowering the concentration of a solution by adding solvent to it is called dilution.…

Q: 3. You have a sucrose solution of 0.047 grams/ml. You want to prepare 100 milliliters of sucrose…

A: In the lab, the stock solution is there, which is of high concentration we can not use that…

Q: Which of the following techniques can best separate a protein from other proteins on the basis of…

A: Gel electrophoresis techniques are routinely used to separate molecules based on size and molecular…