**Chromatogram Analysis for Compound Separation**The image presents chromatograms depicting the separation of compounds A and B. The separations were achieved at the same flow rate using two different columns of equal length. The void time, \( t_M \), is 1.75 minutes for each column.**Graph Explanation:**- **Axes:** - *Vertical Axis (Y-axis):* Detector Response - *Horizontal Axis (X-axis):* Time (minutes), ranging from 0 to 25 minutes.- **Plots:** - *Column 1:* Displays two peaks, with compound A appearing first followed closely by compound B. - *Column 2:* Shows a similar pattern of two peaks for compounds A and B, appearing at different times.**Peak Identification:**- Compound A elutes first in both columns, with its peak appearing before that of compound B.**Questions:**1. **Determine the Unadjusted Relative Retention (\( \gamma \)):** - Calculate the unadjusted relative retention for the two compounds using Column 2.2. **Retention Factor Comparison:** - Which compound has a larger retention factor? - Options: They are the same, compound A, compound B3. **Distribution Constant Comparison:** - Which compound has a smaller distribution constant? - Options: They are the same, compound A, compound BThis analysis is fundamental in understanding the separation dynamics of compounds using chromatography and assists in optimizing separation processes in various analytical applications.

Answered: Image /qna-images/answer/0477b6b4-11c2-4169-b5e8-201d86b90f2b.jpg

Answered: columns of equal length. The void time,…

Science

Chemistry

columns of equal length. The void time, tM, is equal to 1.75 min on each column. A tm Column 1 Column 2 tector Response

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Similar questions

FindTap-Cengage Leaming x b Answered: Determine the pH dur x + tatic/nb/ui/evo/index.html?deploymentid=55750823468596024212874980&elSBN=9781305657571&snapshotid=3054066&id=155276463... ✰ Back Submit Answer Use the References to access important values if needed for this question. Calculate the pH of an aqueous solution that contains 0.453 M sodium hydrogen phosphate and 0.337 M sodium dihydrogen phosphate. Calculate pH of Buffer. ICE Method: This is group attempt 1 of 5 References P W pH = Autosaved at 10:37 PM DE Regenerate Group (40) Next 10:37 11/3/2
A 4.00 (±0.01) mL4.00 (±0.01) mL Class A transfer pipet is used to transfer 4.00 mL4.00 mL of a 0.300 (±0.005) M0.300 (±0.005) M Cu2+Cu2+ stock solution to a 100.00 (±0.08)100.00 (±0.08) Class A volumetric flask. Deionized water is used to bring the flask to volume. I am trying to calculate the concetration of the diluted solution in the volmentric flask and find the absolute uncertainty! Thank you!
A sample of enzyme involved in alcohol metabolism, extracted and purified extensively from yeast cultures, was loaded onto a gel filtration column for molecular weight approximation. A set of protein standards with known molecular weight was eluted separately through the same column and suitable buffer following the elution of blue dextran for void volume determination. The following table summarizes the data obtained from the experiment: Elution flow rate 1 mL/min Sample collector setting Continuous; Sequential Container change rate 1 tube/min Sample/Standard eluted Test tube No/s. MW (Da) Blue dextran 4 2,000,000 Bovine thyroglobulin 11 669,000 Horse spleen apoferritin 14 443,000 Beta-amylase from sweet potato 21 200,000 Bovine serum albumin 27 66,000 Alcohol metabolism enzyme 19 ? 1. Process the given raw data in a tabular format of properly identified data points ready for calibration curve plotting. 2. Construct the calibration curve using MS Excel for use in MW determination.…
given answer with cslculation ...
ACTIVITY 2: Instructions: Identify whether the solution has reached its woH solution process has not yet reached the saturated status. Write your AMA2 A S F enter G H J L caps lock return shift V shift all fn control option command command option Science 7 MODULE 1 saturated status or not. ei uloa bnis tnavloa ers lo srmiev sri 0 olupii Sol the solution process has reached saturated status and put X if the answers in your science activity notebook. nisnos anperoved oilortoole eoM. 1. Mix a pack of tea powder with water in a glass pitcher and stir. The to tea powder dissolved in the water. Gad s ni fonsrds COUCGUCLS 2. Add one tablespoon of sugar in the tea solution and stir. All of thea sugar crystals were dissolved. im 02= nobuloe emulov 3. Add more table spoon of sugar and stir. After stirring, you observed that there were crystals of sugar undissolved. 4. After tasting your tea, you decide it is still not sweet enough and add another table spoon of sugar. After stirring, you…
Calculate the mass/time ratio for the trials at ambient,40,60, and 80 degress
please do this typewritten for upvote
What effect will the following have on plate height (H). Make sure to explain your full and complete reasoning. Increasing stationary phase thickness. Reducing the rate of sample injection. Increasing mobile phase flow rate. Decreasing temperature. Reducing stationary phase particle size.
What is the dimension of a chlorine contact chamber if the CT value is 150mg-min/L at the chlorine concentration 0f 5.0 mg/L. Give the width lengthand depth. Show all work.
Three factors were studied: reactor length, carrier flow rate, and sample volume, with the high and low values summarized in the following table. factor high (+1) level low (–1) level X: reactor length 1.3 cm 2.0 cm Y: carrier flow rate 1.6 mL/min 2.2 mL/min Z: sample volume 100 μL 150μL The authors determined the optimum response based on the greatest sensitivity, as determined by the change in potential for the potentiometric detector. The following table summarizes their optimization results. run X* Y* Z* ΔE (mV) 1 –1 –1 –1 37.45 2 +1 –1 –1 31.70 3 –1 +1 –1 32.10 4 +1 +1 –1 27.30 5 –1 –1 +1 39.85 6 +1 –1 +1 32.85 7 –1 +1 +1 35.00 8 +1 +1 +1 32.15 Which factor has the greatest effect on sensitivity (change in potential at the detector)? Which set of conditions optimizes the detection sensitivity?
the formula they are refering to fot 1/2 time is .693/k. I thought K was slope so I plugged that in and do not know why I got these wrong.
Name the major chemical groups found in organic molecules. Describe the basic structure of each chemical group and outline the chemical properties of the organic molecules in which they occur.