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columns of equal length. The void time, tM, is equal to 1.75 min on each column. A tm Column 1 Column 2 tector Response

columns of equal length. The void time, tM, is equal to 1.75 min on each column. A tm Column 1 Column 2 tector Response

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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**Chromatogram Analysis for Compound Separation** The image presents chromatograms depicting the separation of compounds A and B. The separations were achieved at the same flow rate using two different columns of equal length. The void time, \( t_M \), is 1.75 minutes for each column. **Graph Explanation:** - **Axes:** - *Vertical Axis (Y-axis):* Detector Response - *Horizontal Axis (X-axis):* Time (minutes), ranging from 0 to 25 minutes. - **Plots:** - *Column 1:* Displays two peaks, with compound A appearing first followed closely by compound B. - *Column 2:* Shows a similar pattern of two peaks for compounds A and B, appearing at different times. **Peak Identification:** - Compound A elutes first in both columns, with its peak appearing before that of compound B. **Questions:** 1. **Determine the Unadjusted Relative Retention (\( \gamma \)):** - Calculate the unadjusted relative retention for the two compounds using Column 2. 2. **Retention Factor Comparison:** - Which compound has a larger retention factor? - Options: They are the same, compound A, compound B 3. **Distribution Constant Comparison:** - Which compound has a smaller distribution constant? - Options: They are the same, compound A, compound B This analysis is fundamental in understanding the separation dynamics of compounds using chromatography and assists in optimizing separation processes in various analytical applications.
Transcribed Image Text:**Chromatogram Analysis for Compound Separation** The image presents chromatograms depicting the separation of compounds A and B. The separations were achieved at the same flow rate using two different columns of equal length. The void time, \( t_M \), is 1.75 minutes for each column. **Graph Explanation:** - **Axes:** - *Vertical Axis (Y-axis):* Detector Response - *Horizontal Axis (X-axis):* Time (minutes), ranging from 0 to 25 minutes. - **Plots:** - *Column 1:* Displays two peaks, with compound A appearing first followed closely by compound B. - *Column 2:* Shows a similar pattern of two peaks for compounds A and B, appearing at different times. **Peak Identification:** - Compound A elutes first in both columns, with its peak appearing before that of compound B. **Questions:** 1. **Determine the Unadjusted Relative Retention (\( \gamma \)):** - Calculate the unadjusted relative retention for the two compounds using Column 2. 2. **Retention Factor Comparison:** - Which compound has a larger retention factor? - Options: They are the same, compound A, compound B 3. **Distribution Constant Comparison:** - Which compound has a smaller distribution constant? - Options: They are the same, compound A, compound B This analysis is fundamental in understanding the separation dynamics of compounds using chromatography and assists in optimizing separation processes in various analytical applications.
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