Calculate the concentration of lycopene in your orange fraction using using Beer's law equation A=ExBxC A is the measured absorbance, E is the molar extinction coefficient (M-1cm-1) at a particular wavelength, B is the path length (in cm) and C is the analyte concentration (M).  A= 0.602. Wavelenght is 500.95nm.  Molar extinction coefficients is 123,000M-1cm-1

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter9: Atomic Absorption And Atomic Fluorescence Spectrometry
Section: Chapter Questions
Problem 9.16QAP
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Calculate the concentration of lycopene in your orange fraction using using Beer's law equation

A=ExBxC

A is the measured absorbance, E is the molar extinction coefficient (M-1cm-1) at a particular wavelength, B is the path length (in cm) and C is the analyte concentration (M). 

A= 0.602. Wavelenght is 500.95nm. 

Molar extinction coefficients is 123,000M-1cm-1

 

Absorbance
#
5
6
7
B
orange 50mi sample / 1950 μl hexane
VE2
Sample ID
NM
NM2
INSIGHT Software
Abs
469.67 nm, 0.687 Abs
443.08 nm, 0.463
sqy Z09 0 'wy $6.0093
450 500 550
Wavelength (nm)
600
650
User Name
Administrator
Administrator
Administrator
Date and Time
1/2/2006 2:58 AM
1/2/2006 3:00 AM
1/2/2006 3:02 AM
Administrator 1/2/2006 3:04 AM
Thermo Scientific
Scan
1/2/2006 3:04:06 AM
Sample ID:
VE2
Peaks:
nm
500.95
469.67
443.08
Abs
0.602
0.687
0.463
>
Page 1 of 1
Transcribed Image Text:Absorbance # 5 6 7 B orange 50mi sample / 1950 μl hexane VE2 Sample ID NM NM2 INSIGHT Software Abs 469.67 nm, 0.687 Abs 443.08 nm, 0.463 sqy Z09 0 'wy $6.0093 450 500 550 Wavelength (nm) 600 650 User Name Administrator Administrator Administrator Date and Time 1/2/2006 2:58 AM 1/2/2006 3:00 AM 1/2/2006 3:02 AM Administrator 1/2/2006 3:04 AM Thermo Scientific Scan 1/2/2006 3:04:06 AM Sample ID: VE2 Peaks: nm 500.95 469.67 443.08 Abs 0.602 0.687 0.463 > Page 1 of 1
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