Calculate the concentration of lycopene in your orange fraction using using Beer's law equation A=ExBxC A is the measured absorbance, E is the molar extinction coefficient (M-1cm-1) at a particular wavelength, B is the path length (in cm) and C is the analyte concentration (M). A= 0.602. Wavelenght is 500.95nm. Molar extinction coefficients is 123,000M-1cm-1
Calculate the concentration of lycopene in your orange fraction using using Beer's law equation A=ExBxC A is the measured absorbance, E is the molar extinction coefficient (M-1cm-1) at a particular wavelength, B is the path length (in cm) and C is the analyte concentration (M). A= 0.602. Wavelenght is 500.95nm. Molar extinction coefficients is 123,000M-1cm-1
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Calculate the concentration of lycopene in your orange fraction using using Beer's law equation
A=ExBxC
A is the measured absorbance, E is the molar extinction coefficient (M-1cm-1) at a particular wavelength, B is the path length (in cm) and C is the analyte concentration (M).
A= 0.602. Wavelenght is 500.95nm.
Molar extinction coefficients is 123,000M-1cm-1

Transcribed Image Text:Absorbance
#
5
6
7
B
orange 50mi sample / 1950 μl hexane
VE2
Sample ID
NM
NM2
INSIGHT Software
Abs
469.67 nm, 0.687 Abs
443.08 nm, 0.463
sqy Z09 0 'wy $6.0093
450 500 550
Wavelength (nm)
600
650
User Name
Administrator
Administrator
Administrator
Date and Time
1/2/2006 2:58 AM
1/2/2006 3:00 AM
1/2/2006 3:02 AM
Administrator 1/2/2006 3:04 AM
Thermo Scientific
Scan
1/2/2006 3:04:06 AM
Sample ID:
VE2
Peaks:
nm
500.95
469.67
443.08
Abs
0.602
0.687
0.463
>
Page 1 of 1
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