Calculate the concentration of lycopene in your orange fraction using using Beer's law equation A=ExBxC A is the measured absorbance, E is the molar extinction coefficient (M-1cm-1) at a particular wavelength, B is the path length (in cm) and C is the analyte concentration (M).  A= 0.602. Wavelenght is 500.95nm.  Molar extinction coefficients is 123,000M-1cm-1

Q: Calculate the specific absorbance ratio (SAR), where A620 = 0.166 and A280 = 0.557

A: The specific absorbance ratio (SAR) is calculated as the ratio of the absorbance at 620 nm to the…

Q: 1 2 3 TEST TUBE 4 5 Blank Unknown solution Volume 0.52 M Fe₂(SO4)3 1.00 mL 2.00 mL 3.00 mL 4.00 mL…

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Q: A solution of a specific vitamin has a åmax of 271 nm and a concentration of 2.73 × 10-7M. The…

A: Given that : Concentration of the solution (C) = 2.73 x 10-7 M Absorbance of the solution (A) =…

Q: extinction coefficient = ε, path length = b, concentration C A sample containing an unknown…

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Q: A solution containing a mixture of the compounds X and Y had an absorbance of 0.736 at 443 nm and an…

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Q: Show how the 0.362 in the Absorbance is calculated

A: A question based on absorption that is to be accomplished.

Q: 3. A 2.50×104M solution of a dye has an absorbance of 0.680 when measured in a 1.00 cm cell at 625…

A: The relation between absorbance and transmittance is given by A=2-logT     or A=-logT where A=…

Q: A blue product absorbs light at 590 nm (ε590nm = 7,600 M-1 cm-1). Its concentration was too…

A: The actual concentration is 0.459 mM.

Q: A 2.54 x 10- M sample of compound X in a solvent has an absorbance of 0.370 at 528 nm in a 1.000 cm…

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Q: A 1.3 * 10-5M solution of an unknown substance has an absorbance of 0.345 at its λmax. Calculate the…

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Q: Sally obtains a standard calibration curve for their assigned food dye by plotting absorbance versus…

A: The objective of this question is to determine the concentration of the food dye in the original…

Q: one: Topic Molecular Spectroscopy (16 marks) You were given an unknown mixture that contains two…

A: Step 1: Calculate the absorbance of the mixture at each wavelength (510 nm for salt A and 575 nm for…

Q: Listen A dilute solution of caffeine was analyzed by UV-Visible spectroscopy and found to have a…

A: We can calculate the absorbance factor by two method which are.,  Method 1 :-  The relation between…

Q: C. Spectrophotometry 1. Measure the absorbance of the standard solutions and construct a calibration…

A: A question based on calibration of curve that is to be accomplished.

Q: A standard 3 mL cuvette is two-thirds filled with a 1 in 5 dilution of 70 mM oxyhaemoglobin. The…

A: In the presence of a live yeast suspension, the absorbance is measured to be 0.4, which is 0.2 units…

Q: Analysis of sodium using AA gave the following calibration curve data: A = 0.655 ppmNa %3D Repeat…

A: A = 0.655 ppmNa By the use of AA we will analyze the sodium From the above data, Standard deviation…

Q: A standard of solution was put through appropriate dilutions to give the concentrations of glucose…

A: Straight line equation can be written as:traight line equation can be written as:Using Linear…

Q: An unknown chemical has an absorbance of 0.265 in a 1.0cm cuvette at a concentration of 0.703 M.…

A: From given data A = 0.265  l = 1 cm c = 0.703 M   Formula used A= ԑcl Where, ԑ is absorptivity…

Q: A toluene solution (1.50 mg / L) shows an absorbance of 0.30 in a cuvette with a 0.025 mm optical…

A: The given data contains, Absorbance of toluene = 0.30 Pathlength of cuvette = 0.025 mm.…

Q: For part 2 of the procedure 5 different volumes were prepared of 0.0040 M KMnO4. Table 2- Data for…

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Q: An unknown concentration of [FeSal]+ gave an absorbance reading of 0.301. Calculate the…

A: Given:For the unknown solution:Absorbance = 0.301Slope is 1280 and y-intercept is -0.00821

Q: What absorbance value corresponds to 40.2% T? A = A 0.0130 M solution of a certain analyte exhibits…

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Q: A 6.17 x 10- M sample of compound X in a solvent has an absorbance of 0.300 at 528 nm in a 1.000 cm…

A: Answer: This question is based on Beer-Lambert law which is shown below: A=εbC Here:…

Q: 4.00 3.50 3.00 2.50 2.00 1.50 1.00 y = 19883x + 0.5405 R? = 0.8873 0.50 0.00 0.00E+00 5.00E-05…

A: This can be obtained by putting drop line

Q: Determine the concentration of a solution with an extinction coefficient of 82 Mcm and an absorbance…

A: Use Beer-Lambert's Law.A = ebcwhere A = absorbancee = extinction coefficientb = size of cuvettec =…

Q: 20 What absorbance value corresponds to 47.4% T? A = 0.323 A 0.0141 M solution of a certain analyte…

A: 1. What absorbance value corresponds to 47.4% Т?A =2. A 0.0141 M solution of a certain analyte…

Q: The concentration of phenol in an aqueous solution is 10 ug/mL. The absorbance is found to be 0.209…

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Q: A 131.7 mg sample of powdered Carbamazepine tablets was boiled with ethanol, filtered and then made…

A: Step :

Q: A compound having molecular weight 151.163 g/mol is determined in a sample. A 0.0302 g sample was…

A: Given: Amount of sample dissolved in aqueous solvent = 0.0302 g Molar absorptivity coefficient = 2.6…

Q: The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected…

A: A question based on quantitative analysis, which is to be accomplished.

Q: A chemist received a report from an analysis involving the iron-phenanthroline method, containing…

A: Solutions Hence option (d) is correct answer 37.93ppm

Q: Determining the concentration of unknown solution using uv-vis spectroscopy. The absorbance vs…

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Q: A water sample has been analyzed for phosphate using UVVS method at 662 nm wavelength. The molar…

A: A numerical problem based on Beer-Lambert law, which is to be accomplished.

Q: Graphs are a common data analysis tool used in all branches of science. The given graph represents…

A: The graph between Absorbance and concentration of sample is used to find the concentration of…

Q: The measured absorbance of a solution with a pathlength of 1.00 cm is 0.544. If the concentration is…

A: pathlength (l) =1.00 cm Absorbance (A) = 0.544 concentration(c) = 1.40 x 10 —3M

Q: Compund AFK has a maximum absorbance of 650 nm. Its molar absorptivity at 650 is 8400 M−1cm−1 and…

A: A = εcl  A = absorbance  ε = absorptivity coefficient (M-1cm-1)  c = concentration of solution…

Q: 10 cm 3 air saturated Fricke dosimetry solution in a 1 cm diameter tube is irradiated for 10 min in…

A: Absorbance is a measure of the quantity of light absorbed by a sample. It can be calculated by using…

Q: Maria was tasked to determine the concentration of a Fe2(SO4)3 solution. She prepared 5 different…

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Q: A solution in part B initially contains (4.0800x10^-4) molar SCN" and (2.850x10^-3) molar Fe3+ This…

A: Given that: absorbance = 4.810×10-1  initial concentration of Fe3+ = 4.0800×10-4 M initial…

Q: Draw a plot having X-axis as concentration (mol/L) and Y-axis as absorbance at λ max (525 nm).…

A: 1.  Concentration vs absorbance plot

Q: To determine the content of Pb in a canned sausage, 5.00 g were weighed and after adequate treatment…

A: Beer Lambert's law gives the relationship between absorbance and concentration. THe equation can be…

Q: 33. What is the concentration of an analyte given that the percent transmittance is 70.3%, the path-…

A: Answer: According to Beer-Lambert law A=εbC Here: A=absorbanceb=path lengthC=concentrationε=molar…

Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.