Calculate the amount of GFP in grams produced in the 25-L fermentation used to generate cells for your homogenization lab. To do this problem, recall that for the homogenization step, E. coli cell paste was suspended in Tris buffer, the resulting cell suspension was homogenized. For this calculation, assume that the concentration of GFP in the clarified lysate is 2.0 mg GFP/mL solution. The concentration of cells in the 25-L fermentation was 8% by volume.
Calculate the amount of GFP in grams produced in the 25-L fermentation used to generate cells for your homogenization lab. To do this problem, recall that for the homogenization step, E. coli cell paste was suspended in Tris buffer, the resulting cell suspension was homogenized. For this calculation, assume that the concentration of GFP in the clarified lysate is 2.0 mg GFP/mL solution. The concentration of cells in the 25-L fermentation was 8% by volume.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Calculate the amount of GFP in grams produced in the 25-L fermentation used to generate cells for your homogenization lab.
To do this problem, recall that for the homogenization step, E. coli cell paste was suspended in Tris buffer, the resulting cell suspension was homogenized. For this calculation, assume that the concentration of GFP in the clarified lysate is 2.0 mg GFP/mL solution. The concentration of cells in the 25-L fermentation was 8% by volume.
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