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- Chemistry 5’G-G-C-T-A-T-T-G-A-G•G-A-T-C-C-T-G-G-A-T-G-C-C3’3’C-C-G-A-T-A-A-C-T-C-C-T-A-G•G-A-C-C-T-A-C-G-G5’ a. Give the two DNA fragments generated by the enzyme, and then calculate the binding free energy for re-association of the two, resulting fragments.b. Calculate the equilibrium constant for the formation of the uncleaved complex at 25°C in 1M NaCl.c. Calculate the equilibrium constant for association of the ‘sticky ended DNA’ fragments at 25°C in 1M NaCl.d. Determine the concentration of ds DNA (in part c) after enzyme digestion, if the starting dsDNA concentration was 10-8M.k) Bry FeBrzX Achieve > Students: Register X Assessment - Chem 101 002X nillanlearning.com/courses/55ace5f9-441e-4942-b91a-6dde768c655e/3/k6bu5e/tools/assessment/items/9f6b56a8-92b9-4b ent Check A LGive Up? O Hint OResources Score: 25 Perform the conversions. atm 1.196 atm = 860.8 mmHg = %3D Pa 1386.9 mmHg : atm %3D 287.3 kPa = %3D
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