Please correct answer and don't use hand raiting START8/Determine the plate count of a HPLC column by the half-height method,the tangential method and the 5σ method, using the data supplied in thechromatogram below. Determine the efficiency of the column assumingthat the Theoretical Plate Count for a new column is 3450 plates. What isyour recommendation?WIDTH @ 4.4%52mmWIDTH = 3.5cm47.6cmWIDTH 2½h= 21mm

1. Half-Height Method:N = 5.54 (tR/W1/2h)2 retention time (tR) = 476 mmwidth of the peak at…

Answered: START 8/ Determine the plate count of a…

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter27: Gas Chromatography

Section: Chapter Questions

Problem 27.23QAP

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A chromatogram gives an ideal Gaussian peak with TR = 8.30 min and w1/2 = 0.18 min. How many theoretical plates does the column have? N = What is the plate height of the column in millimeters if the column is 12.8 cm long? H = plates mm
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All types of chromatography depend on partitioning a sample between a stationary phase and a mobile phase. Identify the stationary phase and mobile phase for each of the following types of chromatography. -TLC -GC
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A new method is proposed to simultaneously detectand separatefour foodadditives by reversed-phase liquid chromatography. The data were obtained using theliquid chromatographic column with a flow rate of 0.5 mL per min and with the followingparameters: Pore size 100 A Particle Size 3.5 Internal diameter 4.6 mm Length 150 mm The food additives found in the sample resulted in the following data: ( see image for table) a.Calculate the number of plates from each peak. b.Calculate the plate height for the column. c.Retention factor for each peak.
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Two components in an HPLC separation have retention times thatdiffer by 22 s. The first peak elutes in 10.5 min and the peakwidths are approximately equal. Use a spreadsheet to find theminimum number of theoretical plates needed to achieve thefollowing resolution, Rs, values: 0.50, 0.75, 0.90, 1.0, 1.10, 1.25,1.50, 1.75, 2.0, and 2.5. How would the results change if peak 2were twice as broad as peak 1?
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A spectrophotometric method for the quantitative determination of the concentration ofPb2+ in blood yields an Ssamp of 0.133 for a 1 mL sample of blood that has been diluted to 6 mL. A second sample is spiked with 1 µL of a 1467 ppb Pb2+ standard and diluted to 6 mL, yielding an Sspike of 0.491. Determine the concentration of Pb2+ in the original sample of blood.

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