Please correct answer and don't use hand raiting START8/Determine the plate count of a HPLC column by the half-height method,the tangential method and the 5σ method, using the data supplied in thechromatogram below. Determine the efficiency of the column assumingthat the Theoretical Plate Count for a new column is 3450 plates. What isyour recommendation?WIDTH @ 4.4%52mmWIDTH = 3.5cm47.6cmWIDTH 2½h= 21mm

1. Half-Height Method:N = 5.54 (tR/W1/2h)2 retention time (tR) = 476 mmwidth of the peak at…

Answered: START 8/ Determine the plate count of a…

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter34: Particle Size Determination

Section: Chapter Questions

Problem 34.13QAP

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A chromatogram gives an ideal Gaussian peak with TR = 8.30 min and w1/2 = 0.18 min. How many theoretical plates does the column have? N = What is the plate height of the column in millimeters if the column is 12.8 cm long? H = plates mm
Internal standard. A mixture containing 12.8 mM analyte (X) and 44.4 mM standard (S) gave chromatographic peak areas of 306 for X and 511 for S. A second solution containing an unknown quantity of X plus 55.5 mM S had peak areas of 251 for X and 563 for S. Find [X] in the second solution.
The options are larger or smaller.
Two components in an HPLC separation have retention times thatdiffer by 22 s. The first peak elutes in 10.5 min and the peakwidths are approximately equal. Use a spreadsheet to find theminimum number of theoretical plates needed to achieve thefollowing resolution, Rs, values: 0.50, 0.75, 0.90, 1.0, 1.10, 1.25,1.50, 1.75, 2.0, and 2.5. How would the results change if peak 2were twice as broad as peak 1?
What liquid calculate H for a 50-mm column with N=8,500 plates.
From the data calculate : a) the number of plates from each peak b) the mean and the standard deviation for N c) the plate height for the column
Internal standard (As , Cs ), analyte with known concentration (Ax , Cx ) In a chromatographic equipment, a solution containing 0.006727 X and 0.008331 M S give peak area of Ax= 2316 and As= 207. To analyze an unknown sample, 5.0mL of 0.008331M S was added to 5.0mL of X, and the mixture was diluted to 50.0mL. This mixture gave a chromatography spectrum with area Ax= 3206 and As= 200. Find Cx.
A spectrophotometric method for the quantitative determination of the concentration ofPb2+ in blood yields an Ssamp of 0.133 for a 1 mL sample of blood that has been diluted to 6 mL. A second sample is spiked with 1 µL of a 1467 ppb Pb2+ standard and diluted to 6 mL, yielding an Sspike of 0.491. Determine the concentration of Pb2+ in the original sample of blood.
Pick the HETP (height equivalent to a theoretical plate) listed below that represents the most efficient column. O1 mm/plate O 1 micrometer/plate 1 cm/plate 1 m/plate
Describe with illustrations the observed peak for a chromatogram with the following tailing factors (TF): a. TF = 1b. TF > 1c. TF < 1
During Size exclusion chromatography, if VT = 30 mL, Vs = 15 cm3 , and VL = 15 mL, Vi is 7 mL 8 mL Not enough information 15 mL
A student analyzes sample 1, and sample 2 chromatograms of a mixture containing two compounds (A + B). The following data is obtained: Sample 1: Retention time Area Height % Area 0.210 19.20 15.68 5.57 0.650 243.62 89.37 70.74 1.74 81.57 13.42 23.68 Sample 2: 0.290 64.81 49.90 12.17 0.69 11.61 4.19 2.18 1.95 456.31 43.46 85.66 Using the data from the four compounds below, answer the following questions: Compound (b.p. °C) Retention time Area Height % Area Air 0.280 14.29 8.85 2.81 Hexane (69) 0.666 93.40 33.76 18.34 Cyclohexane (80.7) 0.980 124.66 30.48 24.48 Pentane (98.4) 1.216 112.27 24.18 22.05 Toluene (110.6) 1.830 164.59 21.64 32.32

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