-24. The following data are for a liquid chromatographic column: Length of Packing Flow rate VM Vs A B C D A chromatogram of a mixture of species A. B, C, and D provided the following data: Nonretained Retention Time, min 3.1 5.4 24.7 cm 0.313 mL/min 1.37 mL 0.164 mL 13.3 14.1 21.6 Width of Peak Base (W), min 0.41 1.07 1.16 1.72 Calculate (a) the number of plates from each peak. (b) the mean and the standard deviation for N. (c) the plate height for the column. A B
-24. The following data are for a liquid chromatographic column: Length of Packing Flow rate VM Vs A B C D A chromatogram of a mixture of species A. B, C, and D provided the following data: Nonretained Retention Time, min 3.1 5.4 24.7 cm 0.313 mL/min 1.37 mL 0.164 mL 13.3 14.1 21.6 Width of Peak Base (W), min 0.41 1.07 1.16 1.72 Calculate (a) the number of plates from each peak. (b) the mean and the standard deviation for N. (c) the plate height for the column. A B
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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From the data calculate :
a) the number of plates from each peak
b) the mean and the standard deviation for N
c) the plate height for the column
Transcribed Image Text:L/min. If
the column porosity was 0.43, what was the linear
flow velocity in cm/s?
1-24. The following data are for a liquid chromatographic
column:
Length of Packing
Flow rate
VM
Vs
A
B
C
D
A chromatogram of a mixture of species A. B, C,
and D provided the following data:
Nonretained
Retention
Time, min
3.1
5.4
24.7 cm
0.313 mL/min
1.37 mL
0.164 mL
13.3
14.1
21.6
Width of
Peak Base
(W), min
0.41
1.07
1.16
1.72
Calculate
(a) the number of plates from each peak.
(b) the mean and the standard deviation for N.
(c) the plate height for the column.
*31-25. From the data in Problem 31-24, calculate for A, B,
C, and D
(a) the retention factor.
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