7. Did the digestion of your human 9DNA work? How can you tell? 8. Did the digestion of your PUC19 plasmid work? How can you tell? 9. What size is the digested PUC19 plasmid, according to your gel? To estimate size, simply interpolate based on the two closest bands of the DNA ladder (your lab instructor will give you the known band sizes for this). 10. Does the gel support the average predicted insert size you calculated in Lab 5 (~1600 bp)? Explain your answer. 11. Go back to the table in #3. Suppose you forgot to add the digested human gDNA to the ligation reaction. a. Would you have any ligation taking place? Explain. b. What would this look like if you ran the ligation reaction on a gel? Laboratory 6 Lane 2 Lane 3 Lane 4 Lane 5 base pairs 10000 8000 6000 5000 4000 3500 3000 Lane 1 DNA Ladder Lane 2 Uncut human gDNA (slightly degraded) Lane 3 EcoRI/Hindl-digested human 8DNA 2500 2000 Lane 4 Uncut pUC19 vector 1500 Lane 5 EcoRI/Hindll-digested pUC19 vector 1000 750 500 What is this band? 250 1% agarose gel with GelRed Lane 1

7. Did the digestion of your human 9DNA work? How can you tell? 8. Did the digestion of your PUC19 plasmid work? How can you tell? 9. What size is the digested PUC19 plasmid, according to your gel? To estimate size, simply interpolate based on the two closest bands of the DNA ladder (your lab instructor will give you the known band sizes for this). 10. Does the gel support the average predicted insert size you calculated in Lab 5 (~1600 bp)? Explain your answer. 11. Go back to the table in #3. Suppose you forgot to add the digested human gDNA to the ligation reaction. a. Would you have any ligation taking place? Explain. b. What would this look like if you ran the ligation reaction on a gel? Laboratory 6 Lane 2 Lane 3 Lane 4 Lane 5 base pairs 10000 8000 6000 5000 4000 3500 3000 Lane 1 DNA Ladder Lane 2 Uncut human gDNA (slightly degraded) Lane 3 EcoRI/Hindl-digested human 8DNA 2500 2000 Lane 4 Uncut pUC19 vector 1500 Lane 5 EcoRI/Hindll-digested pUC19 vector 1000 750 500 What is this band? 250 1% agarose gel with GelRed Lane 1

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 1TYK

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Concept explainers

Wound infections

A wound is damaged or breaks in the surface of the skin. The wound is divided into two types, they are acute wound that includes minor cuts, laceration, bites, etc, and chronic wounds that can become slow to heal including ulcers on the legs and feet. Redness, pain, and swelling are signs of a wound.

Question

Questions 7-10 be answered with the image

7. Did the digestion of your human 9DNA work? How can you tell?
8. Did the digestion of your PUC19 plasmid work? How can you tell?
9. What size is the digested PUC19 plasmid, according to your gel? To estimate size, simply
interpolate based on the two closest bands of the DNA ladder (your lab instructor will give you
the known band sizes for this).
10. Does the gel support the average predicted insert size you calculated in Lab 5 (~1600 bp)?
Explain your answer.
11. Go back to the table in #3. Suppose you forgot to add the digested human gDNA to the ligation
reaction.
a. Would you have any ligation taking place? Explain.
b. What would this look like if you ran the ligation reaction on a gel?
Laboratory 6

Lane 2
Lane 3
Lane 4
Lane 5
base pairs
10000
8000
6000
5000
4000
3500
3000
Lane 1
DNA Ladder
Lane 2
Uncut human gDNA (slightly degraded)
Lane 3
EcoRI/Hindl-digested human 8DNA
2500
2000
Lane 4
Uncut pUC19 vector
1500
Lane 5
EcoRI/Hindll-digested pUC19 vector
1000
750
500
What is this band?
250
1% agarose gel with GelRed
Lane 1

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