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7. Did the digestion of your human 9DNA work? How can you tell? 8. Did the digestion of your PUC19 plasmid work? How can you tell? 9. What size is the digested PUC19 plasmid, according to your gel? To estimate size, simply interpolate based on the two closest bands of the DNA ladder (your lab instructor will give you the known band sizes for this). 10. Does the gel support the average predicted insert size you calculated in Lab 5 (~1600 bp)? Explain your answer. 11. Go back to the table in #3. Suppose you forgot to add the digested human gDNA to the ligation reaction. a. Would you have any ligation taking place? Explain. b. What would this look like if you ran the ligation reaction on a gel? Laboratory 6 Lane 2 Lane 3 Lane 4 Lane 5 base pairs 10000 8000 6000 5000 4000 3500 3000 Lane 1 DNA Ladder Lane 2 Uncut human gDNA (slightly degraded) Lane 3 EcoRI/Hindl-digested human 8DNA 2500 2000 Lane 4 Uncut pUC19 vector 1500 Lane 5 EcoRI/Hindll-digested pUC19 vector 1000 750 500 What is this band? 250 1% agarose gel with GelRed Lane 1

7. Did the digestion of your human 9DNA work? How can you tell? 8. Did the digestion of your PUC19 plasmid work? How can you tell? 9. What size is the digested PUC19 plasmid, according to your gel? To estimate size, simply interpolate based on the two closest bands of the DNA ladder (your lab instructor will give you the known band sizes for this). 10. Does the gel support the average predicted insert size you calculated in Lab 5 (~1600 bp)? Explain your answer. 11. Go back to the table in #3. Suppose you forgot to add the digested human gDNA to the ligation reaction. a. Would you have any ligation taking place? Explain. b. What would this look like if you ran the ligation reaction on a gel? Laboratory 6 Lane 2 Lane 3 Lane 4 Lane 5 base pairs 10000 8000 6000 5000 4000 3500 3000 Lane 1 DNA Ladder Lane 2 Uncut human gDNA (slightly degraded) Lane 3 EcoRI/Hindl-digested human 8DNA 2500 2000 Lane 4 Uncut pUC19 vector 1500 Lane 5 EcoRI/Hindll-digested pUC19 vector 1000 750 500 What is this band? 250 1% agarose gel with GelRed Lane 1

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Questions 7-10 be answered with the image
7. Did the digestion of your human 9DNA work? How can you tell? 8. Did the digestion of your PUC19 plasmid work? How can you tell? 9. What size is the digested PUC19 plasmid, according to your gel? To estimate size, simply interpolate based on the two closest bands of the DNA ladder (your lab instructor will give you the known band sizes for this). 10. Does the gel support the average predicted insert size you calculated in Lab 5 (~1600 bp)? Explain your answer. 11. Go back to the table in #3. Suppose you forgot to add the digested human gDNA to the ligation reaction. a. Would you have any ligation taking place? Explain. b. What would this look like if you ran the ligation reaction on a gel? Laboratory 6
Transcribed Image Text:7. Did the digestion of your human 9DNA work? How can you tell? 8. Did the digestion of your PUC19 plasmid work? How can you tell? 9. What size is the digested PUC19 plasmid, according to your gel? To estimate size, simply interpolate based on the two closest bands of the DNA ladder (your lab instructor will give you the known band sizes for this). 10. Does the gel support the average predicted insert size you calculated in Lab 5 (~1600 bp)? Explain your answer. 11. Go back to the table in #3. Suppose you forgot to add the digested human gDNA to the ligation reaction. a. Would you have any ligation taking place? Explain. b. What would this look like if you ran the ligation reaction on a gel? Laboratory 6
Lane 2 Lane 3 Lane 4 Lane 5 base pairs 10000 8000 6000 5000 4000 3500 3000 Lane 1 DNA Ladder Lane 2 Uncut human gDNA (slightly degraded) Lane 3 EcoRI/Hindl-digested human 8DNA 2500 2000 Lane 4 Uncut pUC19 vector 1500 Lane 5 EcoRI/Hindll-digested pUC19 vector 1000 750 500 What is this band? 250 1% agarose gel with GelRed Lane 1
Transcribed Image Text:Lane 2 Lane 3 Lane 4 Lane 5 base pairs 10000 8000 6000 5000 4000 3500 3000 Lane 1 DNA Ladder Lane 2 Uncut human gDNA (slightly degraded) Lane 3 EcoRI/Hindl-digested human 8DNA 2500 2000 Lane 4 Uncut pUC19 vector 1500 Lane 5 EcoRI/Hindll-digested pUC19 vector 1000 750 500 What is this band? 250 1% agarose gel with GelRed Lane 1
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